Colonies

... of electrocompetent e-coli recently and is testing their transformation efficiency. While the cells transform, we are noting a lot of satellite colonies surrounding the main colonies, even where the plates are not that dense in terms of colony numbers. The plates are only grown overnight at ...

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... host cell ) with antibiotic (amp50 for pRSET B, cam30 for pACYCDUET-1) at 37'C overnight. on the next day, i found that only pRSET B have white colonies, but pACYCDUET-1 shows no colonies at all ! what is the possible reason? maybe you can answer it in point (short but easily to understand) ...

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Hallo, You don`t need a serial dilution calculator ... seriously. If you get 55 colonies at a 10^4 dilution, then you have 5.5*10^5 cfu/mL (in case you used an aliquot of 1 mL). If you count 85 colonies at a 10^5 dilution then it`s 8.5*10^6 cfu/mL, which is already ...

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... test them, i plated them out on agar plate containing: Kanamycin, Tetracyclon, Ampicilin an control. After overnight incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately. Since I have kept my original stock of cells before making them ...

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For colony PCR reactions white colonies were picked from the transformation plate. I performed transformation of cloned ANO5 cDNA and the transformation mixture was plated on a LB agar plate containing ampicillin +IPTG+X-Gal. The ANO5 cDNA ...

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