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Collagenase

Collagenase

(Science: enzyme) proteolytic Enzyme capable of breaking native collagen. Once the initial cleavage is made, less specific proteases will complete the degradation.

Collagenases from mammalian cells are metallo enzymes and are collagen type specific. May be released in latent (proenzyme) form into tissues and require activation by other proteases before they will degrade fibrillar matrix. Bacterial collagenases are used in tissue disruption for cell harvesting.


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Extensibility of collagen

... of collagen fibers. I know that infrared radiation has this property, although this effect is light. For example, I'm wondering what would a collagenase liquid do to a connective tissue that would be stretched? Thanks a lot, Raphy

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by Raphy
Tue Nov 03, 2009 9:08 pm
 
Forum: Cell Biology
Topic: Extensibility of collagen
Replies: 7
Views: 3927

Dispersing Agents For Cell Cultures

... find much. I was reviewing some past experimental notes, and we did a similar lab where we tested dispersion abilities of random agents, such as Collagenase, Accutase, HBSS, EDTA, Trypsin/EDTA, and Trypsin themselves. Im looking any other possible "applicable" agents.

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by FusionVisionary
Wed Mar 05, 2008 1:16 am
 
Forum: Cell Biology
Topic: Dispersing Agents For Cell Cultures
Replies: 2
Views: 2826

Tissue digestion for explanted alginate microcapsules???

... to get rid of all the tissue formed around microcapsules, for the protein production assay, but I don't get quite good results. I was told to use collagenase H (2mg/mL) and hialuronidase (1mg/mL) and to keep the tissue embedded in this solution in a water bath 37ÂșC, for three hours... (this was ...

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by Ainhoa M
Wed Jun 06, 2007 5:17 pm
 
Forum: Cell Biology
Topic: Tissue digestion for explanted alginate microcapsules???
Replies: 0
Views: 1161

Adipocytes preparation for FACS (cells from tissue)

I have used trypsin/collagenase solutions to make single cell suspensions... and the cells lived and were healthy. So, i would guess that the trypsin, when done properly will not damage the integrity of the cell membrane. Also, anything ...

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by LilKim
Sun Feb 25, 2007 4:05 am
 
Forum: Cell Biology
Topic: Adipocytes preparation for FACS (cells from tissue)
Replies: 2
Views: 3120

Re: collagenase

Why is collagenase less damaging to cells than trypsin? can comeone please tell me the difference between them. Thanks! collegenase destroys collagen, a part in the connective tissue and it's not in the cell. Trypsine affects ...

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by sdekivit
Tue Aug 09, 2005 3:12 pm
 
Forum: Cell Biology
Topic: collagenase
Replies: 1
Views: 1891
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