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Cohesive end

Definition

noun, plural: cohesive ends

(molecular biology) A fragment of DNA (often produced by a staggered cut on the DNA using restriction enzymes) in which the strands are of unequal length, and an end that is cohesive due to an overhang or a stretch of unpaired nucleotides that easily matches up with another overhang in the complementary region.


Supplement

For example, these two sticky ends can be joined together since they can form complementary base pairs in the overhang region:

5'-ATCTGACT ______+ GATGCGTATGCT-3'
3'-TAGACTGACTACG________CATACGA-5'


Synonym: DNA end, sticky end.
Compare: blunt end.
See also: restriction enzyme, overhang (molecular biology).


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Results from our forum


Re: MOLECULAR CLONING

... Sameer, The ratio between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5256

ligation problem although try everything,suggestions?

... my insert after PCR with the enzymes which my insert have at it's ends (verify again with agarose gel) . Alternatively I cloned my insert to ... I digested my vector with the insert's same enzymes and make sticky(cohesive) end ligation. I used Quick ligase at room temperature(25 degrees) ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4083

dna ligation protocol for cohesive end

Hi, Do anyone suggest me an efficient protocol for cohesive end ligation for cloning? Thank you

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by genetherapy
Thu Jan 26, 2012 7:46 pm
 
Forum: Molecular Biology
Topic: dna ligation protocol for cohesive end
Replies: 3
Views: 1705

Not understanding "rendered flush" concerning vectors

... leave the DNA helix cut at staggered lengths, so they have 'sticky' (cohesive) ends. Although these often make DNA ligation (joining) easier, this only applies if the ends ...

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by merv
Sun Oct 02, 2011 3:03 pm
 
Forum: Molecular Biology
Topic: Not understanding "rendered flush" concerning vectors
Replies: 4
Views: 4606

My water research .. how do you find .!??

... water molecule (H2O) can attract another water molecule, because in the end of the molecule, the oxygen atom has a negative charge, while on the ... has a positive charge and this attractive force is what gives water its cohesive and adhesive properties. Surface Tension As I’ve said before, each ...

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by SWORD MASTER
Tue Oct 24, 2006 10:34 pm
 
Forum: Cell Biology
Topic: My water research .. how do you find .!??
Replies: 4
Views: 1908


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