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Dictionary » C » Codon

Codon

Definition

noun

A set of three adjacent nucleotides, also called triplet, in mRNA that base-pair with the corresponding aniticodon of tRNA molecule that carries a particular amino acid, hence, specifying the type and sequence of amino acids for protein synthesis.


Supplement

For example:

GCC (Guanine-Cytocine-Cytocine) → alanine GUU (Guanine-Uracil-Uracil) → valine CUA (Cytosine-Uracil-Adenine) → leucine UCA (Uracil-Cytosine-Adenine) → serine


Related terms: ambiguous codon, start codon, stop codon.

Synonym: coding triplet.
Compare: anticodon.
See also: pyrimidine, purine.


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Gene cloning question

Thank you. Interesting argument on the extra-Kozak and start codon... So is it even better to leave a few extra amino acids as for a linker in a fusion protein? First I thought the best is if the reporter sequence and "our protein" is right next ...

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by kk
Mon Aug 03, 2009 9:44 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Gene cloning question

you wouldn't want to leave the Kozac sequence and start codon in between the two coding sequences just incase the genes should spit, which there is a chance of if you're trying to splice the gene into a larger sequence or if it is just a plasmid. because ...

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by Eous
Mon Aug 03, 2009 2:57 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Two questions about operon (transcription/translation)

... some policistronic prokaryotic mRNAs have a ribosome binding site (RBS) for each open reading frame. However, others do not: sometimes the last codon of one open reading frame coincides with the first codon of another open reading frame (think AUGA for example) so that when a ribosome has finished ...

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by MrMistery
Sun Aug 02, 2009 11:27 pm
 
Forum: General Discussion
Topic: Two questions about operon (transcription/translation)
Replies: 2
Views: 106

Gene cloning question

... (our "gene" of interest is fused to the N-term to the reporter gene), does it matter if you leave the Kozak sequence and a start codon in between the two coding regions? If it's OK how come that there is no reporter protein being produced alone? Another question: how many extra ...

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by kk
Wed Jul 29, 2009 8:07 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1442

Re: Tricky translation question, can someone help please?

... where regulatory proteins bind the DNA and help a polymerase to hook onto the DNA and start transcription. You need to start with a methionine codon (AUG) and then count triplets (including the methionine) until you reach the stop (which you don't count as an amino acid).

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by jonmoulton
Mon Jun 15, 2009 11:29 pm
 
Forum: Molecular Biology
Topic: Tricky translation question, can someone help please?
Replies: 1
Views: 131
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