Coating

this article may help: http://www.sciencedirect.com/science/article/pii/S0079670007000512 cheers ... Chris

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... antibody, but the wash afterward is causing the pattern to come off. I've tried washing with PBS as well as DI water. I have also tried various coatings for the slide, such as glutaraldehyde, silane derivative and agarose. The only acceptable patterning results are with bare slide. But I'm trying ...

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Does anyone have any experience coating magnetic beads with small, bioactive compounds? Specifically, I am trying to coat Invitrogen Dynabeads (Amine or Carboxylic Acid activated) with vitamin B12 (cobalamin) or B1 (thiamine). I am trying ...

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It is most likely the coating of your coverslips is not good. The lower transfection rate is due to the lower survival rate of your transfected cells on poor coating. I know someone uses coated coverslips from neuvitro.com and got good ...

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... are grown in suspension. I am having trouble fixing the cells and subsequent staining because the cells do not attach to a surface (I have tried coating a glass slide with poly L lysine, but it didn't work). Are there any other ways to either attach the cells to a slide or to stain for actin ...

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