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Coa transferases

Coa transferases

Thiaphorases;enzymes transferring coa from acetyl-coa or succinyl-coa to other acyl radicals.


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Re: problem with cloning/restriction digest

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

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by ashlie23
Sat Feb 09, 2008 5:02 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9027

Re: problem with cloning/restriction digest

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

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by blcr11
Fri Feb 08, 2008 3:38 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 9027

DNA mismatch repair

according to 'Biochemistry' 5th edition by Berg et al it is the cytosine that is methylated on the C-5 by methyl transferases. I am also familiar with methylated adenine to avoid cleavage by restriction endonucleases.

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by sdekivit
Sat Feb 03, 2007 9:02 pm
 
Forum: Molecular Biology
Topic: DNA mismatch repair
Replies: 3
Views: 720

The Fiber Disease

... Each enzyme is assigned a recommended name and an enzyme Commission (EC) number. They are divided into six main groups, oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. Any of several complex proteins that are produced by cells and act as catalysts in specific ...

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by al
Sun Dec 24, 2006 4:08 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749197

The Fiber Disease

... in some detail. The theory states that external magnetic fields can control biological spin-spin proton-proton coupling constants in DNA, RNA, RNA-transferases, and hydrons (H2)). Such spin-spin coupling constants can be stabilized at equilibrium, against the destabilizing external ELFm frequencies. ...

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by Nadas Moksha
Tue Dec 12, 2006 5:23 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749197
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