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Co-immobilised enzyme

Co-immobilised enzyme

(Science: biochemistry) a group of enzymes which work together and are physically confined while doing so.


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Please guide me about these restriction enzymes

you must select enzyme that cut your plasmid in one (or two) determined sequense and also not your target gene.

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by hematologym
Mon Mar 17, 2014 11:17 am
 
Forum: General Discussion
Topic: Please guide me about these restriction enzymes
Replies: 2
Views: 188

Re: Re:

... mix together above 10 c it creates an explosive chemical What exactly is the 10 c? Your theory is nice, but catalase is far from being the only enzyme removing H2O2, there are plenty of various peroxidases. Even if not, H2O2 is highly reactive, so it would react with anything, especially the ...

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by Coelacanth
Sat Feb 22, 2014 10:36 pm
 
Forum: General Discussion
Topic: [News] Frank Baker survives ''spontaneous combustion''
Replies: 5
Views: 726

Re:

... mix together above 10 c it creates an explosive chemical What exactly is the 10 c? Your theory is nice, but catalase is far from being the only enzyme removing H2O2, there are plenty of various peroxidases. Even if not, H2O2 is highly reactive, so it would react with anything, especially the ...

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by JackBean
Tue Feb 18, 2014 9:09 am
 
Forum: General Discussion
Topic: [News] Frank Baker survives ''spontaneous combustion''
Replies: 5
Views: 726

The effect of ethanol on peroxidase activity

... activity increased when i increased ethanol concentration in my lab. I thought it was suppose to be the opposite since alcohol denatures the enzyme...

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by wayne2824
Fri Feb 14, 2014 10:44 pm
 
Forum: General Discussion
Topic: The effect of ethanol on peroxidase activity
Replies: 1
Views: 232

A dilution question about RNA

... in 50 uL water. I ve measured the concentration and it was 3313 ng/uL (Around 165 ug). Now my next step is DNase treatment. It says 1 unit of enzyme for 1 ug of RNA in 10 ul reaction mix. I would like to dilute my RNA to 100 ug and use 100 unite enzyme in 1000 uL reaction mix. Is this logic ...

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by golgicik
Mon Feb 03, 2014 6:30 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 313
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