Cmv

some examples: West Nile virus , Yellow fever virus and for molecular biologists probably most known CMV .

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... I do double digestion in this specific promoter and pEGFP-c1 at the same time, then link the specific promoter into pEGFP-C1, replace the cmv. the problem is after transfection NO GFP expressed! WHY this happen? which step is wrong? help me!thanks! Someone in my lab has been having the ...

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... I do double digestion in this specific promoter and pEGFP-c1 at the same time, then link the specific promoter into pEGFP-C1, replace the cmv. the problem is after transfection NO GFP expressed! WHY this happen? which step is wrong? help me!thanks!

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... type and have been trying to transfect my cells and generate single cell stable clones. The problem is that the gene is under the control of the CMV promoter and we are getting constitutive silencing, this even happens when using a GFP reporter, where there is no phenotype. We can maintain expression ...

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I have cloned something with IRES-EGFP under CMV promoter to generate transgenic mouse. When I checked transfection efficiency of the clone in vitro, I got only 20% cells expressing GFP. Is it necessary to get high expression of the gene in vitro ...

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