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Cloning vector



A piece of DNA where foreign DNA fragment is inserted through recombinant DNA technology.


Cloning vectors are of different types. The most common type is the genetically engineered plasmids and bacteriophages.

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Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, ...

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by miibay
Tue Aug 19, 2014 10:47 pm
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 1786

Re: Gene cloning question

... sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a start codon? (NOTE: My gene is eukaryotic) Is there anyone make a sugestion?

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by mohajeri
Thu Feb 06, 2014 10:01 pm
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 20098

cDNA cloning and Lentiviral transfection

... I have this mcherry labelled integrin cDNA in a mammalian expression vector. If my understanding is correct, I can transfect cells with this vector ... with cDNA, and obtain copies of it? As in, can I use this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 1390

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1906

Cloning software

... a practical course we are searching for a program that we can use for cloning in silico. Like Clonemanager or VectorNTI. We cannot afford to buy ... runs to reduce waiting time. I tested FastPCR, but that doesn't show a vector map and students like pictures. Who can help me on this? So far I ...

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by citroenboom
Wed Nov 14, 2012 10:50 am
Forum: Molecular Biology
Topic: Cloning software
Replies: 1
Views: 1719
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