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Cloning

Definition

noun

The process of creating an exact copy of a biological unit (e.g. a DNA sequence, cell, or organism) from which it was derived, especially by way of biotechnological methods


Supplement

Cloning can be natural or artificial. Examples of cloning that occur naturally are as follows:

  • vegetative reproduction in plants, e.g. water hyacinth producing multiple copies of genetically-identical plants through apomixis
  • binary fission in bacteria
  • parthenogenesis in certain animals

Making multiple copies by manipulation procedures or biotechnology is an artificial cloning. It can be:

  • molecular cloning, where copies of specific gene fragment are produced
  • cellular cloning, where single-celled organisms with the exact genetic content of the original cell are produced in cell cultures
  • organism cloning, or reproductive cloning, where a multicellular clone is created generally through somatic cell nuclear transfer


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An Overview of Embryonic Stem Cells

... initially created for reproductive, purposes. There is an alternative method for deriving human ES cells, namely, the somatic nuclear transfer, or cloning, which bypasses the need to destroy an embryo produced by the fusion of sperm and egg, which could otherwise develop to term if implanted. Difference ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
 
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by miibay
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Help: Confused with primers in a PCR of a vector?

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by JackBean
Sun May 04, 2014 12:00 pm
 
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Topic: Help: Confused with primers in a PCR of a vector?
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Free online software tools for molecular cloning

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by vladCH
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Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a start codon? (NOTE: My g...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
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