Dictionary » C » Cloning

Cloning

Definition

noun

The process of creating an exact copy of a biological unit (e.g. a DNA sequence, cell, or organism) from which it was derived, especially by way of biotechnological methods


Supplement

Cloning can be natural or artificial. Examples of cloning that occur naturally are as follows:

  • vegetative reproduction in plants, e.g. water hyacinth producing multiple copies of genetically-identical plants through apomixis
  • binary fission in bacteria
  • parthenogenesis in certain animals

Making multiple copies by manipulation procedures or biotechnology is an artificial cloning. It can be:

  • molecular cloning, where copies of specific gene fragment are produced
  • cellular cloning, where single-celled organisms with the exact genetic content of the original cell are produced in cell cultures
  • organism cloning, or reproductive cloning, where a multicellular clone is created generally through somatic cell nuclear transfer


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Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 604

Help: Confused with primers in a PCR of a vector?

It's basically designed so that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
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Views: 2683

Free online software tools for molecular cloning

Hello guys, I have recently launched a website http://www.cloninghelp.com where you can find a couple of browser-based tools facilitating your everyday cloning work. At this time a DNA sequence editor and a restriction analysis tool are available. I have ...

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by vladCH
Sat Feb 22, 2014 8:12 pm
 
Forum: Molecular Biology
Topic: Free online software tools for molecular cloning
Replies: 0
Views: 1329

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a start codon? (NOTE: My g...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
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Topic: Gene cloning question
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cDNA cloning and Lentiviral transfection

... transfection. So my question is, Can I transform E.Coli with the vector with cDNA, and obtain copies of it? As in, can I use this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral transduction of my mammalian cells as it has better efficiency. Is there ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
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