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Cloning

Cloning

(Science: molecular biology) The process whereby clones are established asexually, where cells all genetically identical, to a single ancestor.

in recombinant DNA Technology, the use of dna manipulation procedures to produce multiple copies of a single gene or segment of dna is referred to as cloning dna.

The term covers various manipulations for isolating and establishing clones. In simple systems single cells may be isolated without precise knowledge of their genotype. In other systems partial or complete selection of chosen genotypes can be manipulated with gene Cloning.

in plants the term refers to natural or artificial vegitative propagation.


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cell cloning by limiting dilution

I'm not sure I understand your question.. is this a question for cell synchronization? Cells are technical cloning themselves during division.. if you want single cell clones, try a single cell elution and they'll be synchronized too.

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by jyaron
Sat Sep 05, 2009 10:26 pm
 
Forum: Cell Biology
Topic: cell cloning by limiting dilution
Replies: 1
Views: 31

cell cloning by limiting dilution

hi every body does any body did limiting dilution for creating single cell clones? is it a straightforward way or not? any help or protocol will be appreciated best

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by lalehr
Fri Sep 04, 2009 10:22 am
 
Forum: Cell Biology
Topic: cell cloning by limiting dilution
Replies: 1
Views: 31

Improving transient CHO protein expression

... to have increased the expression by almost double, but this still isn't quite enough. Any other suggestions of something I can do (without extra cloning) to imrpove the expression of the cells?

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by nikinje
Wed Aug 19, 2009 4:41 pm
 
Forum: Cell Biology
Topic: Improving transient CHO protein expression
Replies: 0
Views: 48

Re: Gene cloning question

if you leave the kozak sequence it shouldn't matter, you would just end up with a longer linker. well it all depends: some proteins may require the N- or C- terminus to be able to move around in order to fold right. However, you're probably best off starting with 5-10. Also, most of these signal pep...

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by MrMistery
Sat Aug 01, 2009 3:08 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1452

Gene cloning question

Let me continue with molecular cloning. When you generate N-fusion proteins (our "gene" of interest is fused to the N-term to the reporter gene), does it matter if you leave the Kozak sequence and a start codon in between the two ...

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by kk
Wed Jul 29, 2009 8:07 am
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 11
Views: 1452
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