Dictionary » C » Cloning

Cloning

Definition

noun

The process of creating an exact copy of a biological unit (e.g. a DNA sequence, cell, or organism) from which it was derived, especially by way of biotechnological methods


Supplement

Cloning can be natural or artificial. Examples of cloning that occur naturally are as follows:

  • vegetative reproduction in plants, e.g. water hyacinth producing multiple copies of genetically-identical plants through apomixis
  • binary fission in bacteria
  • parthenogenesis in certain animals

Making multiple copies by manipulation procedures or biotechnology is an artificial cloning. It can be:

  • molecular cloning, where copies of specific gene fragment are produced
  • cellular cloning, where single-celled organisms with the exact genetic content of the original cell are produced in cell cultures
  • organism cloning, or reproductive cloning, where a multicellular clone is created generally through somatic cell nuclear transfer


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Free online software tools for molecular cloning

Hello guys, I have recently launched a website http://www.cloninghelp.com where you can find a couple of browser-based tools facilitating your everyday cloning work. At this time a DNA sequence editor and a restriction analysis tool are available. I have ...

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by vladCH
Sat Feb 22, 2014 8:12 pm
 
Forum: Molecular Biology
Topic: Free online software tools for molecular cloning
Replies: 0
Views: 210

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ATG of vector in Nde1 site as a start codon? (NOTE: My g...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 15689

cDNA cloning and Lentiviral transfection

... transfection. So my question is, Can I transform E.Coli with the vector with cDNA, and obtain copies of it? As in, can I use this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral transduction of my mammalian cells as it has better efficiency. Is there ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 569

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1061

Campylobacter RFLP troubleshooting

I do not have any purpose of cloning.My aim is to type 300 Campylobacter strains molecularly and look for similarity between isolates; in short my aim is molcular typing. I am using RFLP just for that. Not for cloning or for something genetical ...

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by gislab03
Tue May 07, 2013 4:06 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 1480
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