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Clones recombinant


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Re: Experimental evidence for evolution

... of the evolution they ran three replay experiments from the potentiated clones. “Statistical Analysis of the Replay Experiments. All three experiments ... asexual, we can nonetheless imagine testing this criterion by producing recombinant genotypes.….” Why is the question left hanging? Because ...

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by jevg
Fri Sep 10, 2010 9:18 pm
 
Forum: Evolution
Topic: Experimental evidence for evolution
Replies: 103
Views: 176551

Recombination in RNA viruses,Ruzic,Kovac

... transesterication mechanism to explain the in vitro generation of recombinants between RNAs associated with Qb bacteriophage ± a possible exception ... M. & Hahn, B. H. (1998). A comprehensive panel of near-full-length clones and reference sequences for non-subtype B isolates of human immunode®ciency ...

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by anakovacevic
Sat Jun 12, 2010 8:28 pm
 
Forum: Cell Biology
Topic: Recombination in RNA viruses,Ruzic,Kovac
Replies: 2
Views: 34870

HELP PLEASE!

... 2.1 x 104 kb in size and the average fragment insert is 10 kb, how many recombinant clones will you need to make a complete genome library representing 99% of the genomic sequences? ...

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by rsu
Sun Oct 18, 2009 7:09 pm
 
Forum: General Discussion
Topic: HELP PLEASE!
Replies: 1
Views: 562

Re: problem with cloning/restriction digest

... in fact work, and that you just have a very low yield of the desired recombinant in a high background of parental vector, you could screen a large number of clones by colony screening a large number of putative clones hoping to find the one clone ...

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by blcr11
Wed Mar 12, 2008 3:48 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 38589

Re: problem with cloning/restriction digest

... me. It seems that all you recover is vector. In the early days of recombinant DNA, some fragments from mammalian cells or viruses would not ... switch the two controls? It makes no sense for there to be more clones in the no-ligase control. I can’t explain it as anything but a fluke. ...

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by blcr11
Wed Mar 12, 2008 2:32 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 38589
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