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Clone cells

Clone cells

a group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary Fission in prokaryotes. Clone cells also include populations of recombinant dna molecules all carrying the same inserted sequence.


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Help: Confused with primers in a PCR of a vector?

... ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2289

Data analysis: DNA alignment HELP?

As genes encoding cyclin-dependent kinases had been cloned from Schizosaccharomyces pombe, the fission yeast, Saccharomyces cerevisae, the budding yeast, and human cells, investigators working in plant systems designed experiments to determine whether plants ...

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by TToe
Mon Feb 18, 2013 10:40 am
 
Forum: Genetics
Topic: Data analysis: DNA alignment HELP?
Replies: 1
Views: 1824

How to go about creating a stably-transfected cell line

... and I'm new to these boards. I'm using P19 mouse embryonal carcinoma cells in my research, and I differentiate them into neurons using retinoic acid. I wish to create a stable clone expressing a GFP-fusion neurotransmitter receptor, of either NMDA-R or AMPA-R, ...

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by JustinNg
Mon Sep 17, 2012 9:26 am
 
Forum: Molecular Biology
Topic: How to go about creating a stably-transfected cell line
Replies: 1
Views: 1771

Re: question for evolutionary biologist.

... oligonucleotides. The sequenced genome was isolated from a yeast clone. The sequenced clone is sMmYCp235-1. This synthetic genome contains ... transplanted in Mycoplama capricolum subspecies capricolum recipient cells in which the restriction modification system had been inactivated. ...

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by scottie
Thu Sep 13, 2012 1:22 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 27495

Re: Creation of species through programmed evolution

... zygote divides mitotically handing down the same genome to the daughter cells. Ultimately tissues are formed. These tissues are structurally and ... in the body tissues calls into question every other claim like clone, personal genome, etc., to cite but a few. c) Biological program comes ...

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by PAWahid
Thu Jan 26, 2012 7:38 pm
 
Forum: Evolution
Topic: Creation of species through programmed evolution
Replies: 15
Views: 10651
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