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Clone bank

Clone bank --> genomic library

(Science: molecular biology) a collection of dna molecules, derived from restriction fragments that have been cloned in vectors, that includes all or part of the genetic material of an organism.

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The Fiber Disease

... [1] The SCOPE program is, ultimately, a CIA/Defense contractor/World Bank/International Monetary Fund plan to force the corporatization of millions ... the species. Nevertheless, Gabriel considered it "unwise" to clone a toxin gene into a necrotrophic pathogen (such as F. oxysporum). ...

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by Skytroll
Wed Jul 12, 2006 6:12 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6263652

isolation of a target gene from a genome

... have to satrt with. If you know the sequence, you can design a PCr and clone the PCR product. Or make a restriction of the genomic DNA, clone evrything ... there are probably a few sequences of such genes available in gene bank, so use degenerate primers to try to amplify something. Or if you have ...

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by canalon
Fri Nov 18, 2005 10:44 pm
Forum: Genetics
Topic: isolation of a target gene from a genome
Replies: 2
Views: 3048

About getting a genetic sequence

You now have your organisms DNA, a whole bank of Applied Biosystems 3730xl 96-capillary machines and shed loads of money. ... reaction is to chop your DNA into chunks (using resitriction enzymes) and then clone these chunks into a vector of some sort. Plasmids are the simplest but you ...

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by DevGrp
Fri Aug 26, 2005 9:18 am
Forum: Genetics
Topic: About getting a genetic sequence
Replies: 10
Views: 7230

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