Clone

Hi I am going to establish single cell clones from adherent and suspend CHO cells, my colleques said that it isnt so easy do you had any expereince it that?

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Hello, In the early attempts of cloning, it was reported that an attempt to clone a frog using a frog's cell nucleus did not progress beyond the stage of tadpole. Does it mean that the code required to switch the development from tadpole to frog is not present ...

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... so maybe we're safe after all. Secondly, what comes to cross-species cloning, I must say that with current knowledge it is challenging enough to clone an individual even when there is no cross-species element involved, not to mention doing it with cells from different species. However, I think ...

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... buffer, 1 microliter T4 ligase. And it generally works out fine. I can honestly say in the past year of cloning I've gotton maybe one or two clones with insert dimers. Let's put it this way: the probability of getting one good clone as a result of a three-way ligation is about the same as ...

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... After this visual confirmation, I transfected the plasmid into HEK293 cells. Transfection was performed at 5 different occasions and no positive clones were obtained. SInce the expression of the insert has been frustating and unsucessful we decided to cut the entire gene out and insert it into ...

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