Dictionary » C » Clone

Clone

Clone

(Science: cell biology) a propagating population of organisms, either single cell or multicellular, derived from a single progenitor cell. Such organisms should be genetically identical, though mutation events may abrogate this.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Gateway technology

... of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction with the destination vector, therefore obtaining ...

See entire post
by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 604

Help: Confused with primers in a PCR of a vector?

... familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This ...

See entire post
by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2687

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence ...

See entire post
by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 17919

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

See entire post
by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1461

Producing unknown protein

... or cell free expression systems) 3. Synthesize sequence (IDT can do it for you). 4. Ligate into vector (vector depends on the expression system)/clone vector. 3. Transform and/or express ...

See entire post
by Cat
Mon May 20, 2013 1:56 pm
 
Forum: Microbiology
Topic: Producing unknown protein
Replies: 2
Views: 2124
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 19,346 times. 
What links here | Related changes | Permanent link