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Clone

Clone

(Science: cell biology) a propagating population of organisms, either single cell or multicellular, derived from a single progenitor cell. Such organisms should be genetically identical, though mutation events may abrogate this.


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Re:

... many generations. Despite that, some animals are capable of giving birth to members of other species. The birth of the first apparently healthy clone of an endangered species was announced today. Scientists at Advanced Cell Technology (ACT) of Worcester, Massachusetts, say they've got a peppy ...

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by wildfunguy
Tue Feb 19, 2013 3:21 pm
 
Forum: Evolution
Topic: mutations and dependencies
Replies: 17
Views: 852

Data analysis: DNA alignment HELP?

As genes encoding cyclin-dependent kinases had been cloned from Schizosaccharomyces pombe, the fission yeast, Saccharomyces cerevisae, the budding yeast, and human cells, investigators working in plant systems designed experiments to determine whether ...

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by TToe
Mon Feb 18, 2013 10:40 am
 
Forum: Genetics
Topic: Data analysis: DNA alignment HELP?
Replies: 1
Views: 119

overexpression xylanase gene

1) this can have several guidelines, since bacteria have no introns, you can easily clone the gene directly from genomic DNA. This clone into any vector (either one for keeping the gene or directly into overexpression one). Transform the vector into E. coli and express. ...

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by JackBean
Thu Dec 13, 2012 11:43 am
 
Forum: Molecular Biology
Topic: overexpression xylanase gene
Replies: 2
Views: 397

Re:

... there is some small alternative-intron in your exon, which can be thus split why are you using primers only for one exon at a time? Is your aim to clone all variants or are you fine with only some? Well, we initially had 4 sets of primers. I was going to amplify 4 overlapping regions of the gene, ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 881

How to clone a gene with lots of splicing intermediates?

... there is some small alternative-intron in your exon, which can be thus split why are you using primers only for one exon at a time? Is your aim to clone all variants or are you fine with only some?

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by JackBean
Mon Dec 10, 2012 6:10 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 881
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