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Clone

Clone

(Science: cell biology) a propagating population of organisms, either single cell or multicellular, derived from a single progenitor cell. Such organisms should be genetically identical, though mutation events may abrogate this.


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anti-hsp70 antibody

... to detect constitutive hsp70, using SDS-PAGE and WB, and I'm pretty sure I've exposed the insects to sufficient heat stress... I'm currently using clone BRM-22 from Sigma, and I wonder if you have any suggestion of other antibodies I might try to detect inducible hsp70. The blueberry maggot is ...

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by biomart
Wed Mar 04, 2015 9:43 am
 
Forum: General Discussion
Topic: anti-hsp70 antibody
Replies: 2
Views: 414

Gateway technology

... of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression clone of a certain gene? Why can't I use an attL-PCR product or a linearized attL DNA and make a reaction with the destination vector, therefore obtaining ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 1769

Help: Confused with primers in a PCR of a vector?

... familiar with what is going on. We recently ligated a gene into a pBS vector and popped it into some E. coli cells. We looked for positive clones via blue/white screening and isolated/purified the respective plasmid. Then, we carried out a PCR reaction of the pBS-gene (our clone). This ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 4613

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 20053

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1892
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