Dictionary » C » Cleavage site

Cleavage site

Definition

noun, plural: restriction sites

The site in a polynucleotide chain as to where the restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


Supplement

The restriction enzyme recognizes the site as to where it cuts via a specific sequence of nucleotides along the polynucleotide chain. For example, when the restriction enzyme, Eco RI, recognizes the palindromic sequence GAATTC, it cuts between G and A on the top and bottom strands.


Synonyms:


See also: restriction enzyme


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Questions on the Sodium Potassium Pump and Enzymes

... and involves the enzyme and the substrate working at an active site? For example DNA Ligase forms phosphodiester bonds between Okazaki fragments... ... and adding new nucleotides to the RNA Primer. Also, what is Proeolytic Cleavage?

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by JoWillyTso
Mon Nov 05, 2012 5:50 pm
 
Forum: Cell Biology
Topic: Questions on the Sodium Potassium Pump and Enzymes
Replies: 3
Views: 3790

LIC PCR Primer with Signal Peptide?

... is (accession number EFF03255). A SignalP analysis showed where the cleavage site was on the submitted amino acid sequence and where the mature protein began. From then ...

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by stalinsma
Wed Oct 19, 2011 8:17 pm
 
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 2754

LIC PCR Primer with Signal Peptide?

... told when designing my primer that I should remove the signal peptide (cleavage site at the 26th amino acid) which corresponds to removing the first 78 or so nucleotides in ...

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by stalinsma
Wed Oct 19, 2011 6:03 pm
 
Forum: Molecular Biology
Topic: LIC PCR Primer with Signal Peptide?
Replies: 3
Views: 2754

Molecular Genetics Questions!

... links/books, etc. Please explain! Thanks in advance!! 8. What new cleavage site(s) is (are) created when DNA is cut with HindIII, filled in by DNA polymerase I, and ligated? ...

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by prenurse
Wed Jan 26, 2011 7:49 pm
 
Forum: Genetics
Topic: Molecular Genetics Questions!
Replies: 1
Views: 1582

cloning into pEGFP-C1 vector

... in frame to be it expressed as fusion protein :roll: Isn't there some site for protease cleavage?

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by JackBean
Sun Apr 25, 2010 11:37 am
 
Forum: Molecular Biology
Topic: cloning into pEGFP-C1 vector
Replies: 6
Views: 5165
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