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Clean room

Clean room

(Science: cell culture) a room in which contamination is limited to a specific maximum level to facilitate the manufacture of sterile and high purity products, to protect personnel, equipment, and products from microbial contamination and to prevent the escape of hazardous particles into the environment.


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Re: Important unknown nanotech within humans

... 53 —Expertise: A Sandia scientist who helped develop a foam spray to clean up congressional buildings and media sites during the anthrax scare ... She died of asphyxiation after being smothered by a pillow in her hotel room while on vacation in Arizona. Aril 18, 2005: Douglas Passaro, 43 —Expertise: ...

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by vincio
Mon Feb 16, 2009 2:36 am
 
Forum: Molecular Biology
Topic: Important unknown nanotech within humans
Replies: 28
Views: 6293

Re: gel electrophoresis & assays help please

... at 37°C in orbital incubator. [3] Remove 1 ml of each culture into a clean eppendorf and pellet cells by spinning in a microfuge for 2 mins. Discard ... to each resuspended pellet, mix well (using vortex) and leave at room temperature for 5 min. [6] Pellet cell debris by centrifugation in microfuge ...

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by RachBio
Sun Feb 08, 2009 9:26 pm
 
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 869

Re: I need a biologist's help

... 9. What does your average work day consist of? enter into the culture room to check the culture of microorgnisms such as mycoplasma check the calender ... test to do make scheme for my assistant and me do some test(experiment) clean up the test result and analyse 10. What are you currently researching? ...

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by patrickpyb
Thu Dec 18, 2008 6:25 am
 
Forum: General Discussion
Topic: I need a biologist's help
Replies: 3
Views: 595

Negative control not negative in RT-PCR

... have contaminated your reaction. In this case what I would do is: 1- Clean pipetters and thermocycler with slightly acidic solution then rinse ... steps as complete as possible: different set of pipettes, different room to prepare and run PCR; good pipetting techique). If the problem is ...

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by canalon
Wed Jul 16, 2008 1:55 am
 
Forum: Molecular Biology
Topic: Negative control not negative in RT-PCR
Replies: 3
Views: 1720

antibody

a month? Maybe in a "clean room"... Is that the case?

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by MrMistery
Wed Jun 04, 2008 4:58 pm
 
Forum: Human Biology
Topic: antibody
Replies: 12
Views: 2063
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