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Circular reaction

Circular reaction

in sensorimotor theory, the tendency of an organism to repeat novel experiences.

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... polynucleotide kinase treatment will participate in the ligation reaction with both their ends having -OH groups. The 3'-OH end of the oligo ... on this? Will those nick be gone and will bacteria amplify intact circular dsDNA plasmids? tx

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by kk
Fri Jul 19, 2013 12:50 pm
Forum: Molecular Biology
Replies: 9
Views: 7150

Microbiology help in identifying Gram + rod for my unknown

... tests and the results I ran. What do you think? Cell shape= rod gram reaction= positive Size when done with a mixed gram stain with . aereus is ... with a slight tint of yellow, the colonies formed slightly, raised, circular bumps. Blood plate= I thought was beta hemolytic because it was ...

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by ccapelouto838
Thu May 03, 2012 5:21 am
Forum: Microbiology
Topic: Microbiology help in identifying Gram + rod for my unknown
Replies: 12
Views: 10997

smear after restriction digestion

... you have intact plasmid, you should have only relaxed and supercoiled circular DNA, shouldn't you? Did you try to incubate the plasmid with full reaction except the enzyme?

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by JackBean
Wed Jan 20, 2010 12:06 pm
Forum: Molecular Biology
Topic: smear after restriction digestion
Replies: 23
Views: 27482

M Biology

... Here are the results of all the tests I performed. Colony morphology Circular, Raised, smooth, glistening Cell morphology Rod, Bacillus (single) ... reduction Alot Nitrate Present Oxidase Oxidase Negative Catalase Strong Reaction Coagulase Negative Result, no clot Hemolysis β-Hemolysis Motility ...

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by ottotrac
Wed Apr 22, 2009 4:43 pm
Forum: Microbiology
Topic: M Biology
Replies: 0
Views: 3699

Re: problem with cloning using pTYB2

... worked, only monomer and dimer would be observed. (2). The ligation reaction, the total volume of 30 uL was used according to the protocol from ... two are >= 1kb different so they could not be super coil and open circular. I have never seen this phenomenon before. I attach the pictures ...

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by ripplemm
Thu May 08, 2008 12:25 am
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 4024
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