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Circular dna

Circular dna

(Science: molecular biology) C.f. Linear dna.

Examples: plasmids, bacterial, mitochondrial, chloroplast and some viral genomes.


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How did endosymbionts coordinate replication?

Another question about mtDNA: what is the advantage to/role of having multiple copies of the same circular DNA molecule? Isn't that "too much of a dose" of the same given gene? Any mitochondria ...

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by kk
Fri Jun 06, 2014 12:16 pm
 
Forum: Cell Biology
Topic: How did endosymbionts coordinate replication?
Replies: 9
Views: 4407

The question about TATA sequence flexibility

Good day :) We know that: The TATA box is the sequence in DNA found in the promoter region of genes in archaea and eukaryotes that is ... are stretched and contracted when bending the DNA molecule (in case of circular DNAs)? http://i.imgur.com/wjkrwzw.jpg

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by Fantomas
Sat Aug 31, 2013 10:57 am
 
Forum: Molecular Biology
Topic: The question about TATA sequence flexibility
Replies: 1
Views: 1695

MOLECULAR CLONING

... to mention, that synthesized oligos have 5'-OH and 3'-phosphate ends... DNA ligase removes 3'-phosphates and replaces it with -OH. Thus, synthesized ... on this? Will those nick be gone and will bacteria amplify intact circular dsDNA plasmids? tx

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by kk
Fri Jul 19, 2013 12:50 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5368

Re: Markerless mutants using suicide vector

... difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean by two linear pieces that are homologous to each other? Do you mean using a linear DNA to KO the gene of interest? If so, we had actually successfully applied ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 2669

Restriction enzyme - I think my teacher made a mistake.

The vector is a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes ... PstI and EcoRI. (The chicken gene fragment replacing the smaller segment of plasmid DNA between these restriction enzyme sites). Where is says Pstl, isn't it meant to be Sstl?

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 1505
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