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Circular dna

Circular dna

(Science: molecular biology) C.f. Linear dna.

Examples: plasmids, bacterial, mitochondrial, chloroplast and some viral genomes.


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Re: Markerless mutants using suicide vector

... difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean by two linear pieces that are homologous to each other? Do you mean using a linear DNA to KO the gene of interest? If so, we had actually successfully applied ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 531

Restriction enzyme - I think my teacher made a mistake.

The vector is a circular molecule containing a total of 5730 bp. It has sites for the restriction enzymes ... PstI and EcoRI. (The chicken gene fragment replacing the smaller segment of plasmid DNA between these restriction enzyme sites). Where is says Pstl, isn't it meant to be Sstl?

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by TToe
Sat Nov 24, 2012 12:36 am
 
Forum: Genetics
Topic: Restriction enzyme - I think my teacher made a mistake.
Replies: 1
Views: 447

Re: help please 1

... - All cells require genetic information, and store it in one or more DNA molecules- What does it say require? Why not use that all cells have genetic information? - The bacterial chromosome is circular and is organised into an amorphous structure called a nucleoid that is ...

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by Stephen1993
Wed Feb 22, 2012 11:19 pm
 
Forum: Cell Biology
Topic: help please 1
Replies: 9
Views: 3135

Re: Theories - Origin of Life

... the mitochondria or chloroplasts of the new cell. These have their own circular genome carry out transcription and translation and replicate independently ... the mitochondria’s genetic code is at variance with the standard DNA code. The codon UGA is a stop codon in the standard code, but encodes ...

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by scottie
Mon Jan 30, 2012 3:56 pm
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 548
Views: 534489

Prokaryotic DNA replication

Does prokaryotic DNA replication involve synthesis of an RNA primer? Like for example the circular chromosome of bacteria. In order to start synthesizing a new strand on the template, a primer may be ...

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by Adz795
Tue Jan 24, 2012 8:54 pm
 
Forum: Cell Biology
Topic: Prokaryotic DNA replication
Replies: 3
Views: 1016
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