Dictionary » C » Chromatography

Chromatography

Definition

noun

The process or technique of separating molecules or components in a mixture according to the differential absorption and elution


Supplement

Column chromatography and paper chromatography are two of the common types of chromatography used in laboratory to separate components in a mixture.


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Dating of blood stains

... please please let us know!): ⋅ Try to seperate the HbO2 molecule from the met-HB molecule (the difference is just Fe2+/Fe3+) using ion chromatography (or some kind of chromatography) and detect them. (Maybe an other option would be a DAD, to detect at different wavelenghts.. however ...

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by wptmdoorn
Tue Jan 28, 2014 12:32 pm
 
Forum: Human Biology
Topic: Dating of blood stains
Replies: 1
Views: 2001

protein fractionation

There are several ways how to purify your protein (bulk fractionation, liquid chromatography, electrophoresis). Best is if you have previously published protocol, at least of similar protein. If you have any, you just have to try different approaches and detect ...

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by JackBean
Thu May 09, 2013 2:03 pm
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2872

Interpretation & Troubleshooting on SDS/Native PAGE

... didn't really enter the gel. What percentage was the gel? The bands on SDS-PAGE are pretty faint. How does your sample look now on gel exclusion chromatography? What was source of your protein? I guess it wasn't native enzyme, because then you could hardly have only three bands (unless you've ...

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by JackBean
Sun Dec 16, 2012 10:17 pm
 
Forum: Molecular Biology
Topic: Interpretation & Troubleshooting on SDS/Native PAGE
Replies: 1
Views: 1968

Scientific paper on DNA replication & gel electrophoresis

... 6 minutes. Strands that migrated to the top of the gel indicate a stalled replication fork. This was the bulk reaction. -Then used gel-filtration chromatography to remove all unbound proteins and then readded only the 3 proteins that were known to be necessary during replication. The same procedure ...

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by dekk1525
Sat Sep 08, 2012 11:17 pm
 
Forum: Molecular Biology
Topic: Scientific paper on DNA replication & gel electrophoresis
Replies: 0
Views: 4923

Why dialyse DMEM before protein purification (Ni-Affinity)?

... etc. but somehow I don't get why it is necessary here - what is in DMEM that would make it difficult (impossible?) to run the ni-affinity chromatography? Would the protein precipitate? If yes, why then? I've been using google for the past days with different combinations of keywords and ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2782
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