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... is expressed under the same promoter control ( mir30 motif). shRNAs were tested against both rat and mouse cDNAs in luciferase assay. The infected cells (in both rat and mouse cell lines) were selected on antibiotic. But when I look at the protein expression in the cells by Western blot, there ...

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I'm not sure I understand your question.. is this a question for cell synchronization? Cells are technical cloning themselves during division.. if you want single cell clones, try a single cell elution and they'll be synchronized too.

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MrMistery, That was an altogether masterful job. The best I've seen on this. Appreciate it very much.

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Does each chromosome in a human cell contain one complete DNA molecule, so that there are 46 separate DNA molecules, one for each chromosome? Or does a single DNA molecule continue as an extended filament, running in and out of one chromosome, ...

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Thanks kolean, I learned something today... :) -Dory

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