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Chloroform

Chloroform

a heavy, clear colourless liquid with a strong ether-like odour, once used for anaesthesia, but now obsolete.


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protein extraction from fat breast tissue

I'd probably go for some sort of phenol-chloroform extraction which should effectively remove fats, but I'm not sure about how quantitative it is. But nowadays are available commercial kits. What about to check publications to see what others do?

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by JackBean
Sat May 04, 2013 7:21 pm
 
Forum: Cell Biology
Topic: protein extraction from fat breast tissue
Replies: 1
Views: 1808

Antimicrobial property of Jatropha podagrica

... to do a research about the antimicrobial property of Jatropha podagrica. I have read some article regarding this plant. In the studies they used chloroform and methanol extracts. Are there other ways in which I can extract the "chemicals" of this plant that exhibits antimicrobial property ...

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by biomeeh
Fri Mar 23, 2012 4:11 am
 
Forum: Microbiology
Topic: Antimicrobial property of Jatropha podagrica
Replies: 1
Views: 1351

Isopropanol, Water, and RNA Stability

... a discussion with someone who remarked in passing that RNA stability is higher in isopropanol than in H2O. (I am preparing to perform a phenol:chloroform extraction of RNA, and they recommended - for added stability - that I keep the RNA in isopropanol instead of H2O until I'm ready to use ...

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by cw11
Mon Nov 28, 2011 7:15 pm
 
Forum: Molecular Biology
Topic: Isopropanol, Water, and RNA Stability
Replies: 3
Views: 2647

Problem with transgenic mice

... you the best result. 2. You are using cDNA - does this mean a crude RT prep? You could do a DNA clean up to remove any proteins (a 50:50 Phenol/chloroform say 100-500ul , vortex, spin 10mins, pipette of the aqueous phase to a fresh tube. dispose of phenol carefully according to local rules (evap ...

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by merv
Sun Oct 02, 2011 9:32 pm
 
Forum: Genetics
Topic: Problem with transgenic mice
Replies: 2
Views: 7198

PLease Please I need help with my PCR! Greatly appreciated!

... you the best result. 2. You are using cDNA - does this mean a crude RT prep? You could do a DNA clean up to remove any proteins (a 50:50 Phenol/chloroform say 100-500ul , vortex, spin 10mins, pipette of the aqueous phase to a fresh tube. dispose of phenol carefully according to local rules (evap ...

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by merv
Sun Oct 02, 2011 5:00 pm
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 9794
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