Accordingly, hydrogen ions (protons) are pumped from the mitochondrial matrix to the intermembrane space via the hydrogen carrier proteins while the electrons are transferred along the electron transport chain in the mitochondrial inner membrane. As the hydrogen ions accumulate in the intermembrane space, an energy-rich proton gradient is established. As the proton gradient becomes sufficiently intense the hydrogen ions tend to diffuse back to the matrix (where hydrogen ions are less) via the ATP synthase (a transport protein). As the hydrogen ions diffuse (through the ATP synthase) energy is released which is then used to drive the conversion of ADP to ATP (by phosphorylation).
This theory was not previously well accepted until a great deal of evidence for proton pumping by the complexes of the electron transfer chain emerged. This began to favor the chemiosmotic hypothesis, and in 1978, Peter Mitchell was awarded the Nobel Prize in Chemistry.
See also: chemiosmosis
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... the generation of an electrochemical gradient across a membrane (chemiosmotic hypothesis) as shown in the schematic.. the picture is of a membrane with an ATP synthase with ...
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... than the single enzyme of substrate-level phosphorylation. Enzymes in chemiosmotic synthesis are arranged in an electron transport chain that is ... either the chloroplast or mitochondrion. According to the chemiosmosis hypothesis proposed by Peter Mitchell in 1961, a special ATP-synthesizing ...
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