Chelator

urea denatures proteins, EDTA is chelator (it binds strongly metal ions), however, to my knowledge, they shouldn't affect lipids

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There are going to be limits possibly as to citrate in the medium specifically, although it isn't essential, as citrate acts as a chelator and destabilizes the alginate beads.

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Just google "trypsinization" and you will get most of the information you want to know. EDTA is a standard chelator of (forms a soluble complex with) calcium and magnesium ions, both of which inhibit the enzyme trypsin. Cells themselves contain these metal ions and calcium ...

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TE is TRis EDTA. Tris is a buffer, EDTA is an chelator for divalent cations. Since many DNAse use metallic cations (Mg2+ etc..) as coenzyme, if they are chelated, they will not be able to degrade your DNA during extraction.

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