Dictionary » C » Chelator



noun, plural: chelating agents

A ligand, often an organic compound, which reacts with a metal ion to produce a chelate.



See also: chelation, ligand.

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Urea/EDTA treatment

urea denatures proteins, EDTA is chelator (it binds strongly metal ions), however, to my knowledge, they shouldn't affect lipids

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by JackBean
Sat Jun 18, 2011 10:34 am
Forum: Cell Biology
Topic: Urea/EDTA treatment
Replies: 1
Views: 2225

Limiting factor on organism density in submerged culture?

There are going to be limits possibly as to citrate in the medium specifically, although it isn't essential, as citrate acts as a chelator and destabilizes the alginate beads.

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by Tsathoggua
Sat Jun 19, 2010 6:01 pm
Forum: Microbiology
Topic: Limiting factor on organism density in submerged culture?
Replies: 2
Views: 2473

Cell culture-biotechnology--->HELP!

Just google "trypsinization" and you will get most of the information you want to know. EDTA is a standard chelator of (forms a soluble complex with) calcium and magnesium ions, both of which inhibit the enzyme trypsin. Cells themselves contain these metal ions and calcium ...

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by blcr11
Wed Jan 14, 2009 7:14 am
Forum: Bioinformatics
Topic: Cell culture-biotechnology--->HELP!
Replies: 2
Views: 3882

Bacterial Plasmid DNA extraction

TE is TRis EDTA. Tris is a buffer, EDTA is an chelator for divalent cations. Since many DNAse use metallic cations (Mg2+ etc..) as coenzyme, if they are chelated, they will not be able to degrade your DNA during extraction.

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by canalon
Tue Jun 19, 2007 6:50 pm
Forum: Molecular Biology
Topic: Bacterial Plasmid DNA extraction
Replies: 3
Views: 4090

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