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Charge transfer

Charge transfer --> charge transfer complex

a complex between two organic molecules in which an electron from one (the donor) is transferred to the other (the acceptor), becoming generally distributed throughout the latter; subsequent transfer of a hydrogen atom completes the reduction of the acceptor; such complex's are generally highly coloured and may be so observed, a network of hydrogen bridges at the catalytic centre of certain proteases.

Synonym: charge transfer system.

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Charge transport through DNA

Hello all! Had anybody met papers where charge transfer was described ( not along single DNA but between different single DNA in the double helix ) ...

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by limarodessa
Sun Jul 17, 2011 8:53 am
Forum: General Discussion
Topic: Charge transport through DNA
Replies: 1
Views: 1540

Biochemistry/ Proteins & Amino Acids

... must change between deoxy and oxy states b) it must be negative in charge c) it must have a pKa value that is above 9 d) all of the above e) ... histidine residues that participate, directly or indirectly, in the transfer of O2 from the lungs to the tissues. Of these, the C-terminal histidine ...

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by SimplyMe
Tue Feb 23, 2010 8:32 am
Forum: General Discussion
Topic: Biochemistry/ Proteins & Amino Acids
Replies: 4
Views: 5815

Re: SDS-PAGE help please

... with a certain amount of running buffer (specially formulated to carry charge). The current runs from the top to the bottom of the box pulling the ... at 100-150V, it just depends on the person and the lab I might add. Gel Transfer to Membrane for Western (immunoblot) Next, the gel is remove from ...

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by MJD
Thu Oct 01, 2009 2:58 am
Forum: Bioinformatics
Topic: SDS-PAGE help please
Replies: 6
Views: 13259

Glycine in Western Blot buffers

... faster. With the boundary gone, the protein can migrate by length/charge. I'm no good at pchem so this explanation may be bunk, but I picture ... pushes the protein along. I assume glycine does the same thing in the transfer buffer: push the protein forward onto the membrane through (assuming ...

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by snowcapk
Tue Aug 12, 2008 4:00 am
Forum: Molecular Biology
Topic: Glycine in Western Blot buffers
Replies: 2
Views: 14676

Denaturing agarose gel electrophoresis

... out according to their molecular weight and more-or-less independent of charge or shape. Usually you transfer the gel to a blot and probe the blot with a labeled oligo that recognizes a specific ...

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by blcr11
Fri Sep 21, 2007 8:04 pm
Forum: Molecular Biology
Topic: Denaturing agarose gel electrophoresis
Replies: 8
Views: 5836
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