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Charge exchange

Charge exchange

(Science: radiobiology) phenomenon in which an ion colliding with a molecule (or an atom) neutralises itself by capturing an electron from the molecule or atom, and transforming the molecule or atom into a positive radical or ion.


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Heavy metals, can anyone answer?

... if it’s coherent. Please ask again if you have any questions.) A net charge (positive or negative) can be stored on your body, but it is neutralized by a flow of electrons ( not “positive-negative ion exchange,” as you said) whenever you ground yourself. This is constantly happening ...

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by AstraSequi
Wed Apr 11, 2012 12:07 am
 
Forum: Human Biology
Topic: Heavy metals, can anyone answer?
Replies: 11
Views: 7292

Re:

No, they will hardly efect something like "charge levels", whatever should it be. Then by what mechanism does static ... has to be maintained on the body until a sufficient positive-negative ion exchange (I.E touching a grounded or metal source) is available. Surely electrolytes, ...

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by JoshuaFlynn
Tue Apr 10, 2012 8:49 am
 
Forum: Human Biology
Topic: Heavy metals, can anyone answer?
Replies: 11
Views: 7292

ion exchange chromatography

Hi there, I plan on doing IEC tomorrow and need to dialyze my protein to keep a negative charge...the plan is to dialyze in Tris pH 8 (pi of my protein is 6) does that sound right...I don't have to add NaCl to the buffer right (NaCl is what I'm eluting with)..I seem ...

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by biology_06er
Wed Nov 02, 2011 3:20 am
 
Forum: General Discussion
Topic: ion exchange chromatography
Replies: 1
Views: 519

pI's of 2 proteins

... to the high ratio of basic to acidic residues on the molecule. A cation-exchange column, specifically carboxymethyl (CMC) is used to help purify ... pI of cytochrome c, thus leaving the molecule with an overall positive charge. This positive charge allows it to bind to the CMC column, which has ...

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by mataiquang
Wed Mar 23, 2011 10:53 pm
 
Forum: General Discussion
Topic: pI's of 2 proteins
Replies: 1
Views: 2598

Buffer Considerations when using Anion Exchange (FPLC)

Hi Everyone! Quick question about protein purification using Anion Exchange column, I'm a little new to the equiptmnt and protein purification. ... pH 7.5. So that means in this buffer my protein will have a net positive charge (hence I cannot use anion exchange????) So my question is what about ...

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by dinofernando
Tue Feb 23, 2010 12:01 pm
 
Forum: Molecular Biology
Topic: Buffer Considerations when using Anion Exchange (FPLC)
Replies: 2
Views: 1603
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