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Results from our forumRe:... ( Corydoras catfish, for example) can swallow air from the water's surface and absorb oxygen through their intestinal lining. Lungs evolved as a chamber of the intestine that gradually split off into its own organ. In essence, your thinking is dependent on the organ already existing before it ...
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Bible vs Darwin... ( Corydoras catfish, for example) can swallow air from the water's surface and absorb oxygen through their intestinal lining. Lungs evolved as a chamber of the intestine that gradually split off into its own organ. In essence, your thinking is dependent on the organ already existing before it ...
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YPEN-1 trypsinisation... 200 because the tip is much narrower. If all else fails, that might solve your problem. 4. If the clumps are small, leave them as is. The counting chamber has a huge error anyway. 5. I just thought of this, no idea if it would work: what about putting the cells in a Corning tube and vortexing them? ...
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Re: Proliferation assay... PMA instead of PHA, but as these twomitogens are pretty similar, I don't think this make a huge difference. I am counting my cells on a Neubauer chamber with trypan blue, and I am also using a nucleocounter to get the number of cells/their viability. I wasn't aware of the CFSE light sensitivity. ...
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Proliferation assay... can spoil it. Btw, what methods you use to ensure that your cells have prolified? Do you just check them under a microscope or count them in a chamber, or just look at the colour of your medium? What cells do you use? For example T cells need proper stimulants to make them divide (e.g. PHA ...
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