Cellulase

... enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into one volume ...

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Cellulase alone should have no effect on the growth of E. coli. Recently i worked with yeast which i cultivated in the presence of a nasty hemicellulase/cellulase mix - and that did not affect the yeast growth. However, if ...

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:) [...] Your point being?

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... (exopolysacharide) forming the matrix of E. coli Biofilm is made of cellulose either. And maybe you just observed dust on your slides, with your cellulase buffer having washed away he bacteria? By the way clumps are not biofilm, the best way to observe biofilms is to put a (sterile) glass plate ...

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I know that Cellulase enzymes that break down cellulose and are involved in cell wall breakdown in higher plants, especially during abscission. Produced in large amounts by certain fungi and bacteria. degradation of cellulose microfibrils ...

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