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Cell lines

Cell line

(Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space.

lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised. Some species, particularly rodents, give rise to lines relatively easily, whereas other species do not. No cell lines have been produced from avian tissues and the establishment of cell lines from human tissue is difficult. Many cell biologists would consider that a cell line is by definition already abnormal and that it is on the way towards becoming the culture equivalent of a neoplastic cell.

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Re: Cell culture - no CO2

... I use non-filter flasks for GMK and Vero and filter for all mammalian cell lines we grow in my Lab. I love you guys. Thanx alot.

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by cheryl4ryl
Fri Nov 22, 2013 12:35 pm
Forum: Cell Biology
Topic: Cell culture - no CO2
Replies: 10
Views: 21236

Passage number

... There are different opinions about this but primarily it depends on the cells that you are growing. In differentiated cell lines the cells wont get reclocked at all. In the lab I work in, we dont consider that the ...

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by jwalin
Fri Aug 30, 2013 2:26 pm
Forum: Cell Biology
Topic: Passage number
Replies: 1
Views: 841

Relationship between cell lines and cancer research (IHC)

... wrong section. At my current internship I am going to stain different cell lines (examples: HELAp2, COLO741, etc) with antigens (immunohistochemistry) relating to cancer ...

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by wptmdoorn
Tue Aug 27, 2013 11:39 am
Forum: Cell Biology
Topic: Relationship between cell lines and cancer research (IHC)
Replies: 2
Views: 858

Physical recovery?

I was thinking more scientifically than that, something along the lines of increasing cell reproduction rate...

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by simpy3
Mon Dec 03, 2012 7:16 pm
Forum: Human Biology
Topic: Physical recovery?
Replies: 5
Views: 2390

Animal cell nucleus shape changes

... can I assume that: 1) Nucleus shape is not fixed, it changes during cell division (mitosis). 2) Under normal condition (not during cell division), ... interested, I can quote my lecture slides. It is not long, just a few lines in fact. Thank you for your feedback.

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by ericjoe
Mon Nov 12, 2012 1:44 pm
Forum: Cell Biology
Topic: Animal cell nucleus shape changes
Replies: 7
Views: 9772
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