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Cell line

Cell line

(Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space.

lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised. Some species, particularly rodents, give rise to lines relatively easily, whereas other species do not. No cell lines have been produced from avian tissues and the establishment of cell lines from human tissue is difficult. Many cell biologists would consider that a cell line is by definition already abnormal and that it is on the way towards becoming the culture equivalent of a neoplastic cell.


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Re: MEG-01 cell line cultivation Problem!!!

I think I have the same problem with MEG 01. I defrost them 3 days ago and they are with many fragments suspended in media with some attached cells. I did not notice an obvious in growth until today. This is the first time I work with MEG 01. How could I differentiate If my cells are dying or this i...

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by Enaam
Fri Oct 03, 2014 9:11 am
 
Forum: Cell Biology
Topic: MEG-01 cell line cultivation Problem!!!
Replies: 1
Views: 1320

use of a bacterial protease inhibitor cocktail with mammalia

... more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical ... (human umbilical vein endothelial cells) and HT-29 adenocarcinoma cell line. Dr Robert Weinberg Dept of Biology, MIT email: rweinber@mit.edu fax: ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 391

STR analysis transfected cells

I stably transfected a population of MCF-7 cells with a pcnda Her2 vector ( I'll call these MCF7-Her2) and now just need to compare my derived cell line with the parent MCF-7 cells by microsatellite STR analysis. Since it is just an addition ...

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by kingmcb
Thu Aug 21, 2014 11:13 pm
 
Forum: Molecular Biology
Topic: STR analysis transfected cells
Replies: 0
Views: 346

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription ...

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 693

Living or not? (viruses,viroids and prions).

... its host, the viral genome carried a lot of genes that replaced basic cellular functions, including some involved in DNA repair and the manufacturing ... viruses' genomes. So, when did the common ancestor exist? The authors line up a few of the conserved megavirus genes (including those of a more ...

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by Sumanth001
Sun Dec 08, 2013 12:37 pm
 
Forum: Microbiology
Topic: Living or not? (viruses,viroids and prions).
Replies: 15
Views: 11248
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