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Cdnas

cDNA --> complementary dna

(Science: molecular biology) dna that is synthesised from a messenger rna template, the single-stranded form is often used as a probe in physical mapping to locate the gene or can be cloned in the double stranded form.

viral reverse transcriptase can be used to synthesise dna that is complementary to rna (for example an isolated mRNA).

Acronym: cDNA


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qPCR

1. extracted RNA --> DNase treatment --> RT to synthesize cDNAs 2. use same primers to amplify your target by PCR 3. extract bands from gel and purify target DNA 4. use the purified DNA for making a standard curve. 5. runa a qPCR

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by bravebeaker
Wed Dec 26, 2012 4:47 am
 
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 2032

Primers Tm

... and 65.7 after both temps no bands occured on the 2% gel. Most important observation for me was changing the template DNA sample using different cDNAs acquired from two different treatments in the study. So now I am thinking of using genomic DNA as a template instead of cDNA. and how about increasing ...

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by bravebeaker
Mon Oct 15, 2012 10:46 am
 
Forum: Molecular Biology
Topic: Primers Tm
Replies: 7
Views: 2757

PCR with one primer? is that possible?

hie everyone, im a student currently taking genetic course, i have to deal with lots of PCR stuffs. Recently, i got completed cDNAs from RACE PCR from my senior, i will like to amplify the full lengh of 3' end of my gene of interested. I wonder if i could use only one gene specific primer(forward ...

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by kyleenyeoh
Fri Feb 05, 2010 4:38 am
 
Forum: Molecular Biology
Topic: PCR with one primer? is that possible?
Replies: 4
Views: 2268

Gene Expression

What's exome? All exones? Just look for CDSs or mRNAs/cDNAs ;)

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by JackBean
Wed Jan 27, 2010 9:51 pm
 
Forum: Genetics
Topic: Gene Expression
Replies: 13
Views: 5688

How can I work out the amount of DNA coding in a region

... you can sequence and identify the coding regions. What organism do you have it from? If is it sequenced, you should be able to find some list of cDNAs or something like that. Also, if you found some sequence of your gene in form of mRNA or cDNA or CDS, you will have the coding region ;) Of course, ...

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by JackBean
Mon Jan 04, 2010 3:54 pm
 
Forum: Genetics
Topic: How can I work out the amount of DNA coding in a region
Replies: 4
Views: 1446
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