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Referring to positively charged ions and their properties.

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Re: Electrophoresis of ionic complexes

... is done at 100V or higher (buffer is virtually the same, but there are no reducing agents or detergents). Just in case anyone is wondering, the cationic and anionic moiety is protonated amine (polymer) and phosphate (siRNA), respectively. I can't imagine voltage may be too influential but I ...

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by dhkwak
Wed Jan 05, 2011 5:55 pm
Forum: Molecular Biology
Topic: Electrophoresis of ionic complexes
Replies: 6
Views: 3989

Re: Transcription inhibition

... Complex. J Infect Dis. 2010 May 3. [Epub ahead of print] Mellbye BL, Weller DD, Hassinger JN, Reeves MD, Lovejoy CE, Iversen PL, Geller BL. Cationic phosphorodiamidate morpholino oligomers efficiently prevent growth of Escherichia coli in vitro and in vivo. J Antimicrob Chemother. 2009 Nov ...

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by jonmoulton
Thu May 27, 2010 3:53 pm
Forum: Molecular Biology
Topic: Transcription inhibition
Replies: 2
Views: 4114

Buffer Considerations when using Anion Exchange (FPLC)

The retention doesn't depend only on overall charge (pI), but also on local cationic/anionic patches, thus your protein can be retained even at pH 7.5 But even if not, the other proteins can be retained and thus your protein of interest purified. That's my case, ...

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by JackBean
Tue Feb 23, 2010 1:13 pm
Forum: Molecular Biology
Topic: Buffer Considerations when using Anion Exchange (FPLC)
Replies: 2
Views: 1809

Discussion of Cocamidopropyl betaine

... it is an allergen.[2][3][4] It also has antiseptic properties, making it suitable for personal sanitary products. It is compatible with other cationic, anionic, and nonionic surfactants. Cocamidopropyl betaine to a significant degree has replaced cocamide DEA. Cocamidopropyl betaine is the ...

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by vikasm
Sat Jul 11, 2009 7:15 am
Forum: General Discussion
Topic: Discussion of Cocamidopropyl betaine
Replies: 0
Views: 4171

Re: Important unknown nanotech within humans

... be made to exist within the biological system Once again we move on to the subject of chemtrails.as previously described the coagullation of the cationic liquid can be achieved by many methods,these methods are strangly present in chemtrials. We have already established that fibres and polymers ...

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by vincio
Tue Feb 10, 2009 7:22 am
Forum: Molecular Biology
Topic: Important unknown nanotech within humans
Replies: 32
Views: 176069
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