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Cathode

Cathode

(Science: chemistry, physics) The electrode in an electrochemical cell toward which cations are drawn and where reduction occurs.

The negatively charged electrode in a vacuum tube.


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BN-PAGE of purified membrane associated protein

... time I used buffer containing coomassie brilliant blue (CBB) G250. Later I read that it is not necessary for purified proteins. The CBB of the cathode buffer is supposed to be enough to charge the protein. And in fact I do not see any differences between the the loading buffer containing CBB ...

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by Papaver
Thu Nov 17, 2011 7:40 am
 
Forum: Molecular Biology
Topic: BN-PAGE of purified membrane associated protein
Replies: 7
Views: 2397

Re: Running SDS PAGE for the first time!

Hi, welcome :) there are several protocols. I have used one with only one running buffer, but last time I have tried other, where were different cathode and anode buffer ;) Actually cathode buffer can be used in subsequent run as anode buffer (or vice versa?). I think, that 50 ug will be plenty ...

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by JackBean
Mon Feb 01, 2010 9:00 pm
 
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 5435

Running SDS PAGE for the first time!

... I am running SDS PAGE shortly and had a few quick questions: Is a running buffer and loading buffer the same thing? If so, are they used as cathode and anode buffers? I have read that cathode and anode buffers are two separate solutions, however some sources suggest that they can be the ...

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by dhkwak
Mon Feb 01, 2010 7:52 pm
 
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 5435

Polyacrylamide gel running

... and the fast chloride ion (in the gel). If Chloride ions are present in the buffer this effect is diminished. 2)The buffer is electrolysed at cathode and anode (remebmber the bubbles at the electrodes?) which changes the pH of the buffer. So never reuse the buffer in SDS-gel electrophoresis. ...

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by andyde
Thu Dec 06, 2007 1:43 pm
 
Forum: Molecular Biology
Topic: Polyacrylamide gel running
Replies: 5
Views: 6555

The Fiber Disease

... to biotech ruling idiot and their we can save the world but we gotta screw 4000 of you up to do so.... from x-rays, to plasmas and their poison cathode....this doc. has it all. and Hey, dammit, I did not write the document nor did i produce it in the http://www.....it was open to the public....except ...

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by London
Tue Dec 12, 2006 10:14 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5560343
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