Cassette mutagenesis

... have to digest out any fragments unless you’re doing some kind of cassette-style transfers to other vectors. It does rely on methylated/unmethylated ... your fragment of interest into donor vector—and you could also do your mutagenesis in the donor as well—you can transfer to any number of pre-designed ...

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... of Tn917-lac transposon into the adcR gene was identified by transposon mutagenesis, which produced a biofilm-defective phenotype. The adcR gene ... operons in S. gordonii was inactivated using a spectinomycin antibiotic cassette and confirmed by PCR. The adcR::specR, adcC::specR, adcB::specR, ...

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