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Cassette mutagenesis

Cassette mutagenesis

The production of mutants within a region (often bounded by unique restriction sites) by the use of synthetic oligonucleotides that fill the gap with mutants designed into the synthetic genetic material.

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New to research and confused! Help? :-)

... have to digest out any fragments unless you’re doing some kind of cassette-style transfers to other vectors. It does rely on methylated/unmethylated ... your fragment of interest into donor vector—and you could also do your mutagenesis in the donor as well—you can transfer to any number of pre-designed ...

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by blcr11
Fri Jun 08, 2007 8:32 am
Forum: Molecular Biology
Topic: New to research and confused! Help? :-)
Replies: 5
Views: 4676

The Fiber Disease

... of Tn917-lac transposon into the adcR gene was identified by transposon mutagenesis, which produced a biofilm-defective phenotype. The adcR gene ... operons in S. gordonii was inactivated using a spectinomycin antibiotic cassette and confirmed by PCR. The adcR::specR, adcC::specR, adcB::specR, ...

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by London
Tue Dec 12, 2006 9:31 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6244981

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