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Carrier screening

Carrier screening

Indiscriminate examination of members of a population to detect heterozygotes for serious disorders and counsel about the risks of marriages with other carriers, and by antenatal diagnosis where a married couple are both carriers; often sacrifices precision to simplicity and is most effectively applied to populations known to be at high risk.

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gel purificatino and/or ta cloning issue

... dna. Using these primers I get 4 bands from the pt. dna (as well as the carrier mother). They are 468 bp, 637bp,~900bp, ~1400bp. The 468bp band is ... the vector not the colony pcr, as there really aren't any negatives in screening. I've tried gel purifying all 4 bands and then TA cloning from ...

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by foster033
Fri May 21, 2010 8:01 pm
Forum: Molecular Biology
Topic: gel purificatino and/or ta cloning issue
Replies: 1
Views: 1303

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