Dictionary » C » Calibrate

Calibrate

Calibrate

1. To graduate or standardise any measuring instrument.

2. To measure the diameter of a tubular structure.


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Re: what is gain in fluorescence detector

When you increased the analyte concentration, did the machine calibrate gain before the first cycle?

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by jonmoulton
Fri Oct 12, 2012 2:40 pm
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2705

what is gain in fluorescence detector

Can anyone explain what GAIN in fluorescence detector actually is? I am running real-time PCR and the signal seems too low for detection if I calibrate gain "before the 1st cycle". If I recalibrate in the middle of the run, I see signal. P.S. If you do SYBR Green PCR, please, let me ...

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by Cat
Tue Oct 09, 2012 12:37 am
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2705

ATP Measurement

... for this product. However, I tried to prepare the solution from Japanese material provided with the ket used for this instrument. While I tried to calibrate/calculate the ATP in my required sample I couldn't get values. I don't know how to use this instrument, if anyone is using this product can ...

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by waqarjadoon
Fri May 25, 2012 9:20 am
 
Forum: Microbiology
Topic: ATP Measurement
Replies: 3
Views: 1349

How to measure dry mass?

... So I guess, but that is an educated guess, that the absorbance would be similarly correlated with dry mass. And I suggested that you verify and calibrate that. But I made the assumption that your microalgae would behave similarly as bacteria, and that there is a known optimal wave length that ...

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by canalon
Thu Aug 04, 2011 6:48 pm
 
Forum: Ecology
Topic: How to measure dry mass?
Replies: 17
Views: 23937

How do I zero the CO2 sensors for a cell culture incubator?

... water-jacketed cell culture incubator (model 3326). We do not have documentation (manual). We are trying to zero the CO2 sensor so we can calibrate the incubator for future cell culture work. Does anybody know how to do this?

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by amperro
Thu Jun 09, 2011 7:01 pm
 
Forum: Cell Biology
Topic: How do I zero the CO2 sensors for a cell culture incubator?
Replies: 2
Views: 1662
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