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Calf-bone

Calf-bone

Synonym: fibula.

2. Bone from a calf (young cow) used in orthopaedic reconstruction.


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Why dialyse DMEM before protein purification (Ni-Affinity)?

... with high glucose and L-Glutamin with 5% fetal calf serum and 5% penicillin/streptomycin. Or do you mean anything else?

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by LittleBird
Sun May 20, 2012 5:55 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2690

What does this mean??? Please help

... 10-40 x 10 8 cells/ml ("Cr-labeled ; 30 min, 100 juCi/ml) were incubated with Ly antiserum diluted in phosphate-buffered saline with 5% fetal calf serum (PBS-FCS) for 1/2 h at 37°C, washed once, brought up in 1 ml of freshly thawed rabbit serum (diluted 1 :8 in PBS), and incubated for a further ...

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by speakers2
Sun Oct 16, 2011 1:06 am
 
Forum: General Discussion
Topic: What does this mean??? Please help
Replies: 0
Views: 2118

Re: genomic rescue construct from BAC DNA

... but the BamHI is reduced to 50-100% activity. What company are the enzymes from? One thing you can do is treat one of your linear fragments with Calf Intestinal Alkalyn Phosphatase (CIP). This will stop re-ligation of your fragment but don't do both fragments (and deactivate the one in the mix) ...

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by antonyviridae
Wed Sep 07, 2011 8:33 pm
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 3870

Re:

Yes replying to enarees is something that I do just to keep my blood pressure high. ... I left you for a while and you made a golden calf. :wink:

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by enarees
Thu Mar 03, 2011 12:37 pm
 
Forum: Human Biology
Topic: AIDS – a tritium disease?
Replies: 23
Views: 13364

Re: weight lifting, repetitions & evolution theory

... muscles you "practice" with. This allows for the flexibility of adapting to different environments — some environments call for stronger calf muscles, others for stronger biceps, etc. (Of course, I am also not an expert in the area of fitness, so I could just be spouting nonsense there. ...

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by Lenoxus
Mon Apr 26, 2010 12:08 am
 
Forum: Evolution
Topic: weight lifting, repetitions & evolution theory
Replies: 6
Views: 8564
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