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Calf

Calf

Synonym: sural region.

2. A young bovine animal, male or female.

Origin: gael. Kalpa


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Re: Human Collembola Infestations

... my body in diatamaceious earth and went to bed. The next day I could SEE these creatures crawling under my skin. There were small bumps on my calf, moving aroundL I could see AND feel them. Convinced I had scabies, I went to the Emergency of a hospital and told them what I thought I had. The ...

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by sammygirl
Thu Feb 05, 2015 2:16 pm
 
Forum: Human Biology
Topic: Human Collembola Infestations
Replies: 47
Views: 132374

Why dialyse DMEM before protein purification (Ni-Affinity)?

... with high glucose and L-Glutamin with 5% fetal calf serum and 5% penicillin/streptomycin. Or do you mean anything else?

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by LittleBird
Sun May 20, 2012 5:55 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3991

What does this mean??? Please help

... 10-40 x 10 8 cells/ml ("Cr-labeled ; 30 min, 100 juCi/ml) were incubated with Ly antiserum diluted in phosphate-buffered saline with 5% fetal calf serum (PBS-FCS) for 1/2 h at 37°C, washed once, brought up in 1 ml of freshly thawed rabbit serum (diluted 1 :8 in PBS), and incubated for a further ...

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by speakers2
Sun Oct 16, 2011 1:06 am
 
Forum: General Discussion
Topic: What does this mean??? Please help
Replies: 0
Views: 2580

Re: genomic rescue construct from BAC DNA

... but the BamHI is reduced to 50-100% activity. What company are the enzymes from? One thing you can do is treat one of your linear fragments with Calf Intestinal Alkalyn Phosphatase (CIP). This will stop re-ligation of your fragment but don't do both fragments (and deactivate the one in the mix) ...

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by antonyviridae
Wed Sep 07, 2011 8:33 pm
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 4376

Re:

Yes replying to enarees is something that I do just to keep my blood pressure high. ... I left you for a while and you made a golden calf. :wink:

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by enarees
Thu Mar 03, 2011 12:37 pm
 
Forum: Human Biology
Topic: AIDS – a tritium disease?
Replies: 23
Views: 15818
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