C banding

... that you know gives the right product size? My lab recently had an issue with contamination in our SuperScript mix that was giving us unwanted banding in our gels and it turned out that our primers were fine. Maybe you could try ordering a primer for a different section of the gene? We usually ...

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... if high or low ionic strength prevents binding completely. You can also try different IMAC resins to see if there are differences in the banding patterns, again suggesting that they could be non-specifically bound contaminants.

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... it was because my proteins are secreted protein, we know for 2-hybrid, the reporter genes can only be motivated when the two proteins of interest banding to the AD and BD separately enter into the nuclear. so maybe the 2-hybrid has some limits I think. For the Co-IP, we need anti-bodies for the ...

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... OD600 got to something like 0.7-1 and then add CAM and go overnight. (This was back in the days when if you needed a lot of DNA you had to do CsCl banding. I don't miss those days and methods at all!) This trick doesn't work with all bacterial strains--and it won't work if your plasmid carries ...

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... I used EcoRI and HindIII to verify the insert based on the size of bands generated. I.E. I am fairly certain the insert is present based on the banding pattern generated from cutting the pBS-insert with EcoRI and HindIII. I actualy asked if XhoI was used on the insert and my suprivisor assured ...

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