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C banding

C banding

(Science: molecular biology) method of defining chromosome structure by staining with giemsa and looking at the banding pattern in the heterochromatin of the centromeric regions. Giemsa banding (g banding) of the whole chromosome gives higher resolution. Q banding is done with quinacrine.


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western blotting of DNA-binding protein of mammalian cell

... and 2X sample buffer added; to the pellet 50ul of 2X sample buffer added, boiled for 5 min in 95C and SDS-PAGE... duing SDS-PAGE in not getting a banding pattern instead i got full smear of gel....simultaneously i have added other sample they ran properly but not this cell lysate... what could ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 420

Re: Codons are triplets, but what's a Singlet, Doublet, etc?

... While the GPU technology is coming of age I’m working on the problems that do not require a supercomputer to solve. The chromosome banding program is one of them. It does not need to search for similarity like the BLAST algorithm does, this algorithm just has to most simply dissect ...

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by GaryGaulin
Thu Oct 25, 2012 2:15 am
 
Forum: Bioinformatics
Topic: Codons are triplets, but what's a Singlet, Doublet, etc?
Replies: 4
Views: 14555

Codons are triplets, but what's a Singlet, Doublet, etc?

... program (a simple how-to for programmers and general public) that compares/displays chromosomes, using a technique I called "digital banding". Here is the illustration for Orangutan, Gorilla, Chimp, Human Chromosome 2. https://sites.google.com/site/digitalchromosomebanding/home/RevComplimentTripletAbundancesForChr2OrangutanGorillaChimpHuman900Bands150kBasesBrightness20.png ...

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by GaryGaulin
Tue Oct 23, 2012 2:55 am
 
Forum: Bioinformatics
Topic: Codons are triplets, but what's a Singlet, Doublet, etc?
Replies: 4
Views: 14555

Re: Basic electrophoresis digest banding pattern analysis....

I'm no mol bio person but why is the uncut control (2nd lane) running as two bands so far apart? Isn't plasmid supposed to run in three bands pretty close to each other? Student A (lane 3) looks as if he has (i) Forgot to add enzyme/added too less. (ii) Added way too much plasmid. The later seems mo...

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by whizliz
Wed May 04, 2011 10:03 am
 
Forum: Molecular Biology
Topic: Basic electrophoresis digest banding pattern analysis....
Replies: 5
Views: 4501

Basic electrophoresis digest banding pattern analysis....

I want to know more about banding pattern analysis...... lane1: standard makers (HindIII digest, sizes are 23.1,9.4,6.6,2.3,0.6,0.1kb) lane2: undigested plasmid X lane3: plasmid X with restriction enzyme SciI by student A lane4: plasmid ...

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by scholesmu
Thu Apr 28, 2011 12:44 am
 
Forum: Molecular Biology
Topic: Basic electrophoresis digest banding pattern analysis....
Replies: 5
Views: 4501
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