CFU

Hallo, You don`t need a serial dilution calculator ... seriously. If you get 55 colonies at a 10^4 dilution, then you have 5.5*10^5 cfu/mL (in case you used an aliquot of 1 mL). If you count 85 colonies at a 10^5 dilution then it`s 8.5*10^6 cfu/mL, which is already close to the concentration ...

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Then I think I should just ignore the cfu and convert Mol to mMol, because it clearly specifies that the concentration is 3.39 mol/cfu. Thanks a lot. :)

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Then it's technically per CFU, because each cell theoretically give rise to single colony. On the other hand, the concentration inside cell will be different from that in media.

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The problem is, I want to use the concentration value somewhere else and I want it to be specified in millimoles. Is it okay if I just ignore the cfu and use the value as millimol? Will it remain same? I just want the concentration of ATP inside the bacterial cell.

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you have to keep it in mmol/CFU

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