Buffers

just weight all chemicals, put them into one beaker and let dissolve. That's how you make buffers.

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... of interest have high GC content or plenty of hairpins, specific conditions should be applied. In case of normal PCR it means special buffers, DMSO/BSA, etc. 4. Chealating agents. Don't know if any of your solutions have them. If 'yes', Mg2+ concentration should be adjusted.

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Look any DGGE protocol. In my experience it does not save time at all. And neither does it saves n reagents, but buffers are cheap anyway.

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I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?

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... with either 10 mM imidazol or 50 mM imidazol) and in the end eluted (250 mM imidazol). I know that a dialysis is in general necessary if buffers need to be changed, one wants to get rid of some certain ingredients/contaminations/salts etc. but somehow I don't get why it is necessary here ...

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