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Buffers

(noun) Plural form of buffer.

See buffer for definition and additional information.


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Taq Buffer ?

why Taq DNA Polymerase Buffers containing Gelatin ?

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by Helics
Sat Jan 18, 2014 9:36 am
 
Forum: Molecular Biology
Topic: Taq Buffer ?
Replies: 1
Views: 574

electrophoretic mobility shift assay (EMSA)

... I experience is that while at first, there was a bromophenol blue front formed during native PAA gel elfo, and since then (using the same buffers, equipment, settings) I don't see the blue front line at all! Also, the RioRad gel running tank shuts down every few minutes saying "arcing ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1218

The same old silly question about molarities

just weight all chemicals, put them into one beaker and let dissolve. That's how you make buffers.

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by JackBean
Wed Jan 09, 2013 9:42 am
 
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 1592

PCR from cDNA question

... of interest have high GC content or plenty of hairpins, specific conditions should be applied. In case of normal PCR it means special buffers, DMSO/BSA, etc. 4. Chealating agents. Don't know if any of your solutions have them. If 'yes', Mg2+ concentration should be adjusted.

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by dustman
Mon Jun 25, 2012 2:09 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3133

restriction reaction on agarose gel slices

Look any DGGE protocol. In my experience it does not save time at all. And neither does it saves n reagents, but buffers are cheap anyway.

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by canalon
Wed Jun 06, 2012 1:18 am
 
Forum: Molecular Biology
Topic: restriction reaction on agarose gel slices
Replies: 2
Views: 1511
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