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Dictionary » B » Buffer BufferDefinition noun, plural: buffers (1) (chemistry) A buffer solution : a solution containing either a weak acid and a conjugate base or a weak base and a conjugate acid, used to stabilize the pH of a liquid upon dilution. (2) (biochemistry) An ionic compound that when added to a solution neutralizes both acids and bases without significantly changing the original acidity or alkalinity of a solution. (3) (ecology) A buffer species: a plant or animal species that becomes an alternate prey to a predator that typically feeds on another species. (4) Anything that cushions, shields or protects against harm, damage, etc., e.g. a high reproductive rate is an efficient buffer against population crashes.
In ecology, a buffer species is a plant or an animal that becomes an alternate prey to a predator. This happens when the predator's preferable, usual prey species is scarce or absent. A buffer may also refer to a vegetated area near a stream that plays a conservatory role in the environment. Such buffer is particularly called a riparian buffer. In biochemistry, a buffer, specifically a buffer solution, is essential for many biochemical processes. It serves to maintain the correct pH necessary as many enzymes work only under precise pH conditions.
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Results from our forumseparation problem with GelRed... not even be a problem with gelred. So why not post stain? If this is really not an option, lowering voltage is usually a good start. And re-using buffer less.
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separation problem with GelRed... bromide to Gelred in our lab. (We always prestain gels - poststaining is no option because its too inconvenient and timeconsuming - using 1xTAE buffer). Our DNA samples and the ladders aren't separated as sharply as it was when we used ethidium bromide. I already have some ideas what to try ...
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Cell counting questionTris is just a buffer http://en.wikipedia.org/wiki/Tris it does not lyse cells. It's basically an environment the cells are happy in because it has the right pH (your TA probably used Trist pH=7.4). The anomaly you witnessed was ...
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Western Blot from an Agarose gel...?Hello all, i made a 4% agarose gel for protein separation using 1x tgs buffer. I was wondering if it would be pointless (or even detrimental) to perform a Western blot on the same gel, just to visualize it better. help? Thanks
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Calculation - Help PLEASEHi, I have a PCR buffer with MgCl2 at 15mM. I have an additional solution of MgCl2 at 25mM. Using this solution, I would like to increase the MgCl2 concentration of my PCR buffer to 50mM. How much of this solution do I need to add ...
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