Dictionary » B » Buffer



noun, plural: buffers

(1) (chemistry) A buffer solution : a solution containing either a weak acid and a conjugate base or a weak base and a conjugate acid, used to stabilize the pH of a liquid upon dilution.

(2) (biochemistry) An ionic compound that when added to a solution neutralizes both acids and bases without significantly changing the original acidity or alkalinity of a solution.

(3) (ecology) A buffer species: a plant or animal species that becomes an alternate prey to a predator that typically feeds on another species.

(4) Anything that cushions, shields or protects against harm, damage, etc., e.g. a high reproductive rate is an efficient buffer against population crashes.


In ecology, a buffer species is a plant or an animal that becomes an alternate prey to a predator. This happens when the predator's preferable, usual prey species is scarce or absent.

A buffer may also refer to a vegetated area near a stream that plays a conservatory role in the environment. Such buffer is particularly called a riparian buffer.

In biochemistry, a buffer, specifically a buffer solution, is essential for many biochemical processes. It serves to maintain the correct pH necessary as many enzymes work only under precise pH conditions.

Word origin: from obsolete buff, to make a sound like a soft body being hit, of imitative origin.

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Results from our forum

RNA isolation from brain sections

... 1) any suggested RNA isolation kits/protocols for brain 2) suggestions for such small volumes of RNA 3) viability of RNA sectioned in cold buffer on a vibratome Overall I'd love to get a nice protocol together that optimizes my RNA recovery for qPCR analysis. Any and all suggestions are ...

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by protoemics
Fri Mar 20, 2015 2:19 am
Forum: General Discussion
Topic: RNA isolation from brain sections
Replies: 0
Views: 36

TAE Buffer preparation

Please how do I prepare 10 M tae buffer containing 2M tris, 0.5M edta, 17.4M acetic acid and adjusted to pH 8.0 with NaOH .

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by dunamis
Thu Feb 26, 2015 6:05 pm
Forum: Molecular Biology
Topic: TAE Buffer preparation
Replies: 0
Views: 298

Methylene Blue Solution use in staining variety of bacteria?

... (toxic shock syndrome). Gram stain experiments are another type of a multi-step staining and de-staining experiment. Reagents 0.1M Sodium Acetate Buffer, pH4.2: Sodium acetate, trihydrate (MW 136.1) ------ 1.36 g Distilled water ----------------------------------- 100 ml Mix to dissolve and adjust ...

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by scytek
Sun Feb 22, 2015 9:22 am
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 267

western blotting of DNA-binding protein of mammalian cell

... one of the DNA-binding protein.... first i have cultured the cells in 25 cm2 flask and collected the cell lysate using RIPA lysis (1 ml) buffer...kept in ice for 5 min, followed by passing the lysate through 24 G needle about 5 times...then centrifuged at 12000 g for 15 min..collected ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 392

separation problem with GelRed

... GelRed. 3. Pour a lower percentage of agarose gel. Higher molecular weight DNA separates better with a lower percentage gel. 4. Change the running buffer. TBE buffer has a higher buffering capacity than TAE buffer. 5. Try using the post-staining protocol if possible, especially if more than the ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19556
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