Dictionary » B » Buffer

Buffer

Definition

noun, plural: buffers

(1) (chemistry) A buffer solution : a solution containing either a weak acid and a conjugate base or a weak base and a conjugate acid, used to stabilize the pH of a liquid upon dilution.

(2) (biochemistry) An ionic compound that when added to a solution neutralizes both acids and bases without significantly changing the original acidity or alkalinity of a solution.

(3) (ecology) A buffer species: a plant or animal species that becomes an alternate prey to a predator that typically feeds on another species.

(4) Anything that cushions, shields or protects against harm, damage, etc., e.g. a high reproductive rate is an efficient buffer against population crashes.


Supplement

In ecology, a buffer species is a plant or an animal that becomes an alternate prey to a predator. This happens when the predator's preferable, usual prey species is scarce or absent.

A buffer may also refer to a vegetated area near a stream that plays a conservatory role in the environment. Such buffer is particularly called a riparian buffer.

In biochemistry, a buffer, specifically a buffer solution, is essential for many biochemical processes. It serves to maintain the correct pH necessary as many enzymes work only under precise pH conditions.


Word origin: from obsolete buff, to make a sound like a soft body being hit, of imitative origin.

Related terms:


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Propagation and Transfection of BHK cells?

... can work with complete media (including FCS) without changing media, high reproducibility because Viromers form stable particles of 300-400nm in buffer. If you are interested I may send a free sample to you. We provide three transfection reagents: for siRNA (Viromer BLUE, BLACK and GREEN) and ...

See entire post
by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1141

Overexpression

... can work with complete media (including FCS) without changing media, high reproducibility because Viromers form stable particles of 300-400nm in buffer. If you are interested I may send a free sample to you. We provide two transfection reagents: for siRNA (Viromer BLUE and GREEN) and for plasmids ...

See entire post
by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1441

The effect of ethanol on peroxidase activity

... concentrations 2) you added the ethanol to yest, not to proteins 3) what was in that solution? Was it some growth medium or was it just water/buffer/whatever?

See entire post
by JackBean
Mon Feb 17, 2014 12:43 pm
 
Forum: General Discussion
Topic: The effect of ethanol on peroxidase activity
Replies: 1
Views: 801

Re: Western transfer failure

Check the transfer buffer ingredients too, I had this transfer problem once when I forgot to add SDS in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went ...

See entire post
by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 4945

Re: A dilution question about RNA

... in 1ml??? :shock: You would take around 30 ul of your RNA (100ug / 3.3ug/ul), add the DNase worth few tens of bucks at least, 100 ul of 10x buffer and water up to 1 ml.

See entire post
by JackBean
Thu Feb 06, 2014 4:31 pm
 
Forum: Molecular Biology
Topic: A dilution question about RNA
Replies: 1
Views: 712
View all matching forum results

This page was last modified 11:46, 5 February 2009. This page has been accessed 99,894 times. 
What links here | Related changes | Permanent link