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(Science: abbreviation) bovine serum albumin.

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Taq Buffer ?

Does it? I've never heard about it. However, my guess would be it's some sort of filling agent, like if you used BSA or something similar.

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by JackBean
Sat Jan 18, 2014 2:36 pm
Forum: Molecular Biology
Topic: Taq Buffer ?
Replies: 1
Views: 575

preparation of Carboxymethyllysine (CML)

Hi, Preparation of CML as a major Advanced Glycation Endproducts in the lab is too critical, we usually use BSA and glyoxalic acid, and we add NaCNBH3 to the mixture. Does anyone know why we add NaCNBH3 to prepare CML? Thanks.

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Thu Jan 02, 2014 3:41 pm
Forum: Cell Biology
Topic: preparation of Carboxymethyllysine (CML)
Replies: 1
Views: 1415

The same old silly question about molarities

... the enzyme solution used in protoplast isolation, well in the paper its written like this 20 mM MES 0.4 M mannitol 20 mM KCl 10 mM CaCl2 0.1% BSA 1.5% Cellulase and so on... I know how to prepare each stock solution individually but how can I translate all these molarites and percentages into ...

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by bravebeaker
Wed Dec 26, 2012 3:35 am
Forum: Molecular Biology
Topic: The same old silly question about molarities
Replies: 3
Views: 1594

Animal-Derived Laboratory Reagents

... blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include: FBS (fetal bovine serum) & BSA (bovine serum albumen): I figured these products are likely a byproduct of the slaughterhouse industry and therefore the animals are being slaughtered ...

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by southtowns18
Thu Dec 06, 2012 6:13 pm
Forum: General Discussion
Topic: Animal-Derived Laboratory Reagents
Replies: 0
Views: 1136

DNase I stability

... linearly. And yes, proteins are more stable in crowd, just think about how it looks in the cell. That's why one need to use balast proteins like BSA in some cases.

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by JackBean
Tue Nov 06, 2012 10:01 am
Forum: Molecular Biology
Topic: DNase I stability
Replies: 1
Views: 1480
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