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Broth

Broth

in the food industry, a thin liquid which is made by boiling meat or vegetables or bones and is used in preparing other foods. It can be found in powder form in most grocery stores.

(Science: cell culture) a liquid medium containing a variety of nutrients which is used to grow cultures of bacteria and other microorganisms.


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MolBio calculations

... 0.25 micrograms of plasmid DNA was added to 400 microliters of competent cells. The mixture was heat shocked and added to 4.5 milliliters of L Broth. Subsequently 50 microliters of the L Broth was plated onto medium which selects for cells carrying the plasmid and the following day 256 colonies ...

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by nedal
Wed Dec 04, 2013 12:32 pm
 
Forum: Molecular Biology
Topic: MolBio calculations
Replies: 1
Views: 863

URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... so much GRAM NEGATIVE- clustered in chains, splotches, and red/pink. Blue Vegetative cells. SPORE REACTION (-) ACID FAST STAINING (-) NUTRIENT BROTH CULTURE- Quite turbid, slightly cloudy, no skin NUTRIENT AGAR STREAK- Shape – Cocci, Yellow, 0.2-0.5m Glossy NUTRIENT AGAR STAB: Aerobic GELATIN ...

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by microbiologyhelp
Wed Nov 20, 2013 6:09 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 2604

Re: Veal infusion agar/broth

Is that veal infusion agar still available. I have a lab regarding effectiveness of hand washing that calls for it. jmkezele

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by JMKezele
Wed Sep 11, 2013 10:20 pm
 
Forum: Microbiology
Topic: Veal infusion agar/broth
Replies: 3
Views: 2905

indole test reaction

guys, i used Lauryl Tryptose Broth for indole test, since the Techical Data Sheet of LTB says it can be used for such biochemical test ( it has Typtose), i incubated the 5 tubes (with inoculum) at 44.5 degrees and after 24 hrs. i dropped ...

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by magandangbutiki
Fri Aug 02, 2013 7:53 am
 
Forum: Microbiology
Topic: indole test reaction
Replies: 0
Views: 463

CFU

... a student counted the following number of colonies on nutrient agar plates inoculated with 100 l of respective dilutions from an original broth culture of E. coli. 10-5 = Too many to count (TMTC) colonies 10-6 = 248 colonies 10-7 = 33 colonies 10-8 = 1 colony 1. From this data state how ...

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by angel92
Mon Jul 22, 2013 9:42 pm
 
Forum: Molecular Biology
Topic: CFU
Replies: 1
Views: 652
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