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Bromide

Bromide

(Science: chemistry) a compound of bromine with a positive radical.


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Gel Electrophoresis Help!

size of your vector/insert. gel is prestained with ethidium bromide or not. what are the restriction enzymes you are using? did you check if your vector contains multiple sites of the enzymes you are using? The bands are way up, higher than 10k bp..

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by bravebeaker
Thu Aug 29, 2013 7:14 am
 
Forum: Molecular Biology
Topic: Gel Electrophoresis Help!
Replies: 3
Views: 903

PCR not consistent

... control give appropriate band? And agarose gel is also important in visualising the results. You should use freshly prepared agarose and ethidium bromide solution.

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by gislab03
Sat May 04, 2013 2:16 pm
 
Forum: Molecular Biology
Topic: PCR not consistent
Replies: 3
Views: 1658

How to prepare a 3.5% agarose gel ?

... microwave oven on low-medium for a couple of soconds a few times to get rid of the bubbles. and after getting a clear solution i added the atidium bromide. worked great.

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by itamo
Sun Feb 17, 2013 5:21 am
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 1727

DNA extraction from Human blood

1. Too much Ethidium Bromide. 2. If this is supposed to be total DNA, it's heavily degraded. Check for contamination...

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by Cat
Fri Sep 14, 2012 2:58 pm
 
Forum: Molecular Biology
Topic: DNA extraction from Human blood
Replies: 3
Views: 2082

Lipofectin/Labfectin lethality towards insect cells

... that makes them so lethal for insect cells during transfection? -L-alpha-phosphatidylethanolamine, dioleoyl -Dimethyldioctadecylammonium Bromide I understand that we are using a lipid and a molecule that can act as a lipid but what do they do to kill the cells so readily? Other sites ...

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by antonyviridae
Fri Sep 02, 2011 4:17 pm
 
Forum: Molecular Biology
Topic: Lipofectin/Labfectin lethality towards insect cells
Replies: 5
Views: 5037
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