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Blunt end

Definition

noun

(general) The end part (of a body, of a leaf, of a petal, etc.) that has a dull or rounded edge.

(molecular biology) The end of a DNA fragment resulting from the breaking of DNA molecule in which there are no unpaired bases, hence, both strands are of the same length.


Supplement

In molecular biology, a blunt end may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases. An example of a blunt end:

5'-CTGATCTGACTGATGCGTATGCTAGT-3'
3'-GACTAGACTGACTACGCATACGATCA-5'


Related terms: blunt-ended DNA.

Compare: overhang, cohesive end.


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Help: Confused with primers in a PCR of a vector?

... that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2218

TOPO PCR/Cloning

... Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I'm not sure if that mean's I'll have my product chopped along the way ...

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by coppcw08
Wed Nov 14, 2012 5:59 pm
 
Forum: Molecular Biology
Topic: TOPO PCR/Cloning
Replies: 1
Views: 909

Re: MOLECULAR CLONING

... between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation we can make ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5250

ligation problem although try everything,suggestions?

... my insert after PCR with the enzymes which my insert have at it's ends (verify again with agarose gel) . Alternatively I cloned my insert to a cloning vector (did blunt end ligation with cloning vector, verify the ligation with agarose gel) then digested ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4078

Re: Isolation of a Gene of Interest for Subcloning

... exactly what I'm looking for. I was also pointed in the direction of blunt-end cloning.

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by HawkPidgeon
Mon Apr 16, 2012 11:15 pm
 
Forum: Genetics
Topic: Isolation of a Gene of Interest for Subcloning
Replies: 2
Views: 1176
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