Dictionary » B » Blunt-ended dna

Blunt-ended dna

Definition

noun

(molecular biology) A fragment of DNA resulting from the breaking of DNA molecule in which there are no unpaired bases or overhangs in the end, hence, both strands are of the same length.


Supplement

For example:

5'-CTGATCTGACTGATGCGTATGCTAGT-3'
3'-GACTAGACTGACTACGCATACGATCA-5'


Related terms: blunt end.

Compare: overhang, sticky end.


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Help: Confused with primers in a PCR of a vector?

It's basically designed so that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2729

Re:

... That is, the population is undergoing disruptive selection . However, some Homo are starting to make tools. In the grassland, these Homo make blunt weapons. In the forest, these Homo make long-reaching sticks with knifed tips that sever the fruits fromt he branches. If this population can ...

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by wildfunguy
Sat Oct 19, 2013 3:13 pm
 
Forum: Evolution
Topic: Is complexity inevitable?
Replies: 16
Views: 10814

Re: mutations and dependencies

... this idea. Originally, I was thinking in terms of functions with specific requirements and functions with loose requirements. Here's an example: Blunt object can be defined in terms of what it does. A blunt object serves the function of bashing or breaking stuff. Many things meet the requirements ...

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by wildfunguy
Sun Feb 17, 2013 8:33 pm
 
Forum: Evolution
Topic: mutations and dependencies
Replies: 29
Views: 22087

TOPO PCR/Cloning

... Invitrogen p-ET-102 Directional cloning kit. My question is the following: Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I'm not sure if that mean's I'll have my product chopped along the way to create that blunt-end. Anyone ever use this kit ...

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by coppcw08
Wed Nov 14, 2012 5:59 pm
 
Forum: Molecular Biology
Topic: TOPO PCR/Cloning
Replies: 1
Views: 954

Re: MOLECULAR CLONING

Hi Sameer, The ratio between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation we can make up to 100:1 ratio but while we're doing cohesive end insert-vector ligation ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5615
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