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Blunt-ended dna

Definition

noun

(molecular biology) A fragment of DNA resulting from the breaking of DNA molecule in which there are no unpaired bases or overhangs in the end, hence, both strands are of the same length.


Supplement

For example:

5'-CTGATCTGACTGATGCGTATGCTAGT-3'
3'-GACTAGACTGACTACGCATACGATCA-5'


Related terms: blunt end.

Compare: overhang, sticky end.


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ligation problem although try everything,suggestions?

... gel) . Alternatively I cloned my insert to a cloning vector (did blunt end ligation with cloning vector, verify the ligation with agarose ... the protocol said (NEB)alternatively try 25 minutes and also tried T4 DNA ligase at room temperature (NEB). I make 1:1, 1:3, 1:5 and 1:10 ligations. ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4068

GFP fusion protein

... do a mini-prep (Promega makes these and many other companies) and send DNA for sequencing. Make sure that you don't autoclave your LB with ITPG ... is that you don't know if your protein is in correctly. Are you using blunt end or sticky end restriction enzymes to make the cut between GFP and ...

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by daniel.kurz
Mon Nov 21, 2011 2:33 am
 
Forum: Molecular Biology
Topic: GFP fusion protein
Replies: 1
Views: 923

genetic engineering- not understanding some concepts

... i hope I can get answered. Thank you :) 1. When making mRNA to DNA, we use reverse transcriptase. When the cDNA is made and we remove the ... sites for solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you know any sites ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1745

Inserting DNA Fragment into Plasmid

Scenario: You digest DNA into fragments and cut plasmids at a specific site, then mix them together and add ... in the proper orientation (not backwards?) Assume the DNA has sticky ends and not blunt ends. This is just a summary of a question I have to answer, but knowing that the ...

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by NiceOnTheIce
Sun Nov 21, 2010 1:53 am
 
Forum: Molecular Biology
Topic: Inserting DNA Fragment into Plasmid
Replies: 3
Views: 5271

PCR of degraded DNA

... it doesn't work. You would need to do some ligation, but I would expect blunt ends, so it would be unspecific and thus you will get just lot of mess ;) How, that is your DNA degraded?

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by JackBean
Thu Nov 19, 2009 11:37 am
 
Forum: Genetics
Topic: PCR of degraded DNA
Replies: 5
Views: 4147
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