Dictionary » B » Blunt-end ligation

Blunt-end ligation

Definition

noun

The process of joining two blunt ends together using an enzyme ligase.


Supplement

This laboratory technique requires ligase since there are no single-stranded overhanging ends for the attachment to form more spontaneously.


See also: blunt-end.


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genetic engineering- not understanding some concepts

... I was wondering if there are any good sites for solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you know any sites that have answers ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1786

Re: How to joint two cloning genes?

Blunt Ended Ligation?

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by domwood
Tue Apr 12, 2011 3:13 pm
 
Forum: Molecular Biology
Topic: How to joint two cloning genes?
Replies: 3
Views: 2050

Problem with SmaI restriction digest

... a vector by amplifying them and ligating the PCR products into a SmaI(=blunt-ended cutter)-digested plasmid, but I've been having trouble with it ... second time due to the smaller amount of DNA, and when I do the actual ligation and try the transformation with that, I don't get any more colonies ...

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by Orangistae
Sat Apr 24, 2010 5:43 am
 
Forum: Molecular Biology
Topic: Problem with SmaI restriction digest
Replies: 6
Views: 3643

PCR of degraded DNA

... primers, so it's no wonder, it doesn't work. You would need to do some ligation, but I would expect blunt ends, so it would be unspecific and thus you will get just lot of mess ;) How, that is ...

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by JackBean
Thu Nov 19, 2009 11:37 am
 
Forum: Genetics
Topic: PCR of degraded DNA
Replies: 5
Views: 4211


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