Dictionary » B » Blunt-end ligation

Blunt-end ligation

Definition

noun

The process of joining two blunt ends together using an enzyme ligase.


Supplement

This laboratory technique requires ligase since there are no single-stranded overhanging ends for the attachment to form more spontaneously.


See also: blunt-end.


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Re: MOLECULAR CLONING

... changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation we can make up to 100:1 ratio but while we're doing cohesive ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5595

ligation problem although try everything,suggestions?

Hi all, I'm in trouble with ligation nearly 5 months. My vector is 4775 bp. My ... PCR with the enzymes which my insert have at it's ends (verify again with agarose gel) . Alternatively I cloned my insert to a cloning vector (did blunt end ligation with cloning vector, verify the ligation ...

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by genetherapy
Wed May 16, 2012 3:31 pm
 
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 4334

GFP fusion protein

... way that you can check that you've done your ligation successfully. Its the way that I would ... makes these and many other companies) and send DNA for sequencing. Make sure that you don't ... if your protein is in correctly. Are you using blunt end or sticky end restriction enzymes to make ...

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by daniel.kurz
Mon Nov 21, 2011 2:33 am
 
Forum: Molecular Biology
Topic: GFP fusion protein
Replies: 1
Views: 974

genetic engineering- not understanding some concepts

... I was wondering if there are any good sites for solving questions about ligation, restriction endonuclease, blunt and sticky ends and pUC8 plasmids and whatnot. If you know any sites that have answers ...

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by radioinahole
Fri Oct 21, 2011 2:38 pm
 
Forum: Molecular Biology
Topic: genetic engineering- not understanding some concepts
Replies: 2
Views: 1827

Not understanding "rendered flush" concerning vectors

... lengths, so they have 'sticky' (cohesive) ends. Although these often make DNA ligation (joining) easier, this only applies if the ends ... other- as this often not the case, it is necessary to blunt the ends- if Klenow fragment of DNA polymerase is ...

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by merv
Sun Oct 02, 2011 3:03 pm
 
Forum: Molecular Biology
Topic: Not understanding "rendered flush" concerning vectors
Replies: 4
Views: 4705
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