Blunt-end ligation

... that could be helpful. 1) Not that I left my ligase on the desk unattended but about your point 1-" if the ligase has been left on the bench ... words how would the CIP help, wouldnt using CIP make more sense in blunt end producing enzymes? I had followed points 4 and 6. Thanks for -Point ...

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... help with molecular cloning (directional, not blunt end). I've been going at this for 5 months, and I believe ... at room temperature (this is according to protocol on ligation mixture) at 3:1 or 1:1 ratio 4) Transform via either ...

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... thread. The intein questions are near the end of it: http://biology-online.org/biology-forum/about12887.html ... well near the ends of linear DNA. I think the ligation didn't work, and my first guess would be ... ligation is going to be a mixed sticky-end and blunt-end ligation. You could try ...

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... method by IMPACT-CN. NdeI (producing sticky end) and SmaI (blunt end) restriction sites were constructed into primers ... insert (gene) and the vector were used to perform ligation with T4 DNA ligase. Since the expected product ...

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... rate of nucleic acids. •Improves efficiency of end-labeling with T4 Polynucleotide Kinase(1,2). •Used in ligation of blunt ended DNA. •Precipitates bacteriophage ± particles and plasmid ...

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