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Dictionary » B » Blunt-end

Blunt-end

Definition

(general) The end part (of a body, of a leaf, of a petal, etc.) that has a dull or rounded edge.

(molecular biology) The end of a DNA fragment resulting from the breaking of DNA molecule in which there are no unpaired bases, hence, both strands are of the same length.


Supplement

In molecular biology, a blunt-end may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases. An example of a blunt end:

5'-CTGATCTGACTGATGCGTATGCTAGT-3'
3'-GACTAGACTGACTACGCATACGATCA-5'


Related terms: blunt-ended DNA.

Compare: overhang, cohesive end.


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Re: Ligation and expression trouble

... that could be helpful. 1) Not that I left my ligase on the desk unattended but about your point 1-" if the ligase has been left on the bench ... words how would the CIP help, wouldnt using CIP make more sense in blunt end producing enzymes? I had followed points 4 and 6. Thanks for -Point ...

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by solution
Wed Jul 08, 2009 9:16 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 398

pcDNA 4/TO

... much in this but I think it would have been better if you had ends which are not complementary on the plasmid to prevent it from closing on itself. Maybe blunting one end of the plasmid and the corresponding end on the insert and using a ligase ...

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by GreenDog
Tue Jan 13, 2009 10:56 am
 
Forum: Molecular Biology
Topic: pcDNA 4/TO
Replies: 1
Views: 458

Quick Question !

... adaptor before. I think it may have been used to talk about converting blunt-end restriction sites into stick-end'ed restriction sites.. but that has nothing to do with ...

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by Jonesy
Wed Dec 31, 2008 2:53 pm
 
Forum: Genetics
Topic: Quick Question !
Replies: 7
Views: 617

Help please! Directional cloning: colonies with WEIRD DNA?

... all, I desperately need help with molecular cloning (directional, not blunt end). I've been going at this for 5 months, and I believe if I can overcome this hurdle I will ...

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by walkthewok
Sun Nov 30, 2008 5:41 am
 
Forum: Molecular Biology
Topic: Help please! Directional cloning: colonies with WEIRD DNA?
Replies: 1
Views: 550

problem with cloning using pTYB2

... through this rather lengthly thread. The intein questions are near the end of it: http://biology-online.org/biology-forum/about12887.html Your protocol ... is just a guess. Your ligation is going to be a mixed sticky-end and blunt-end ligation. You could try doing a short (2-8 hr) ligation at 30 ...

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by blcr11
Tue May 06, 2008 5:08 pm
 
Forum: Molecular Biology
Topic: problem with cloning using pTYB2
Replies: 3
Views: 1306
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