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Blunt-end

Definition

(general) The end part (of a body, of a leaf, of a petal, etc.) that has a dull or rounded edge.

(molecular biology) The end of a DNA fragment resulting from the breaking of DNA molecule in which there are no unpaired bases, hence, both strands are of the same length.


Supplement

In molecular biology, a blunt-end may result from the breaking of double-stranded DNA; however, there are no overhangs or unpaired bases. An example of a blunt end:

5'-CTGATCTGACTGATGCGTATGCTAGT-3'
3'-GACTAGACTGACTACGCATACGATCA-5'


Related terms: blunt-ended DNA.

Compare: overhang, cohesive end.


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Help: Confused with primers in a PCR of a vector?

... that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2696

Re:

... That is, the population is undergoing disruptive selection . However, some Homo are starting to make tools. In the grassland, these Homo make blunt weapons. In the forest, these Homo make long-reaching sticks with knifed tips that sever the fruits fromt he branches. If this population can ...

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by wildfunguy
Sat Oct 19, 2013 3:13 pm
 
Forum: Evolution
Topic: Is complexity inevitable?
Replies: 16
Views: 10733

Re: mutations and dependencies

... this idea. Originally, I was thinking in terms of functions with specific requirements and functions with loose requirements. Here's an example: Blunt object can be defined in terms of what it does. A blunt object serves the function of bashing or breaking stuff. Many things meet the requirements ...

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by wildfunguy
Sun Feb 17, 2013 8:33 pm
 
Forum: Evolution
Topic: mutations and dependencies
Replies: 29
Views: 22022

TOPO PCR/Cloning

... Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I'm not sure if that mean's I'll have my product chopped along the way ...

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by coppcw08
Wed Nov 14, 2012 5:59 pm
 
Forum: Molecular Biology
Topic: TOPO PCR/Cloning
Replies: 1
Views: 954

Re: MOLECULAR CLONING

... between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation we can make ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5586
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