Dictionary » B » Blunt

Blunt

Blunt

1. Having a thick edge or point, as an instrument; dull; not sharp. The murderous knife was dull and blunt. (Shak)

2. Dull in understanding; slow of discernment; stupid; opposed to acute. His wits are not so blunt. (Shak)

3. Abrupt in address; plain; unceremonious; wanting the forms of civility; rough in manners or speech. Hiding his bitter jests in blunt behavior. A plain, blunt man.

4. Hard to impress or penetrate. I find my heart hardened and blunt to new impressions. (Pope)

blunt is much used in composition, as blunt-edged, blunt-sighted, blunt-spoken.

Synonym: obtuse, dull, pointless, curt, short, coarse, rude, brusque, impolite, uncivil.

Origin: cf. Prov. G. Bludde a dull or blunt knife, dan. Blunde to sleep, Sw. & Icel. Blunda; or perh. Akin to E. Blind.


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Help: Confused with primers in a PCR of a vector?

It's basically designed so that you see both length of your insert and it's direction. Did you use blunt-end cloning? Because otherwise one doesn't need to be so careful about the directions.

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by JackBean
Sun May 04, 2014 12:00 pm
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 2175

Re:

... That is, the population is undergoing disruptive selection . However, some Homo are starting to make tools. In the grassland, these Homo make blunt weapons. In the forest, these Homo make long-reaching sticks with knifed tips that sever the fruits fromt he branches. If this population can ...

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by wildfunguy
Sat Oct 19, 2013 3:13 pm
 
Forum: Evolution
Topic: Is complexity inevitable?
Replies: 16
Views: 9346

Re: mutations and dependencies

... this idea. Originally, I was thinking in terms of functions with specific requirements and functions with loose requirements. Here's an example: Blunt object can be defined in terms of what it does. A blunt object serves the function of bashing or breaking stuff. Many things meet the requirements ...

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by wildfunguy
Sun Feb 17, 2013 8:33 pm
 
Forum: Evolution
Topic: mutations and dependencies
Replies: 29
Views: 20657

TOPO PCR/Cloning

... Invitrogen p-ET-102 Directional cloning kit. My question is the following: Will I be using my entire recombinant protein? It says that I need a blunt-end PCR product but I'm not sure if that mean's I'll have my product chopped along the way to create that blunt-end. Anyone ever use this kit ...

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by coppcw08
Wed Nov 14, 2012 5:59 pm
 
Forum: Molecular Biology
Topic: TOPO PCR/Cloning
Replies: 1
Views: 903

Re: MOLECULAR CLONING

Hi Sameer, The ratio between insert and vector changes whether you have an cohesive (sticky) end insert or a blunt end insert. Generally while we're doing blunt end insert-vector ligation we can make up to 100:1 ratio but while we're doing cohesive end insert-vector ligation ...

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by genetherapy
Mon Jul 16, 2012 3:11 pm
 
Forum: Molecular Biology
Topic: MOLECULAR CLONING
Replies: 9
Views: 5216
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