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Blots

Blots

refers to a number of similar techniques that facilitate the analysis of biological molecules. Initially, mixtures of proteins or dna or rna fragments are separated by electrophoresis through a polymeric gel (most commonly agarose or polyacrylamide). The separated components are then transferred to a membrane where they are immobilised and can be analysed and visualised by a variety of techniques, including antibody binding and dna hyrbridisation. Some of the various types of blots are briefly described below. Southern blot - The gel is a dna electrophoresis system and the molecules transferred are dna molecules. Northern blot - similar to the southern blot, but used to analyse rna. Western blot - The molecules analysed in a western blot are proteins, separated by gel electrophoresis, then identified by reacting them with an antibody. Southwestern blot - this is a variant of the southern blot used to find protein molecules that stick to dna molecules. Dot blot. Dna, rna, or protein are dotted directly onto the membrane support, so that they form discrete spots.


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I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include: FBS (fetal bovine serum) & BSA (bovine ...

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by southtowns18
Thu Dec 06, 2012 6:13 pm
 
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by mutant11
Fri Nov 18, 2011 10:17 pm
 
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BN-PAGE of purified membrane associated protein

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by Papaver
Wed Nov 16, 2011 12:28 pm
 
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Topic: BN-PAGE of purified membrane associated protein
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Western Blotting of small molecular weight

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by Homie7
Thu Sep 08, 2011 11:46 am
 
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Topic: Western Blotting of small molecular weight
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probing mRNA and RT-PCR products

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by SarahTessler
Thu Jun 09, 2011 4:22 am
 
Forum: Molecular Biology
Topic: probing mRNA and RT-PCR products
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